SCGB1A1 ELISA Kit (Human) (OKCD06912)

Catalog No: OKCD06912

Size:96WELLS

SCGB1A1 ELISA Kit (Human) (OKCD06912) Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Clara Cell Protein 16 (CC16). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Clara Cell Protein 16 (CC16). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Clara Cell Protein 16 (CC16), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Clara Cell Protein 16 (CC16) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Clara Cell Protein 16 (CC16) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Clara Cell Protein 16 (CC16) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • SCGB1A1 Microplate: 96 Wells (12 x 8 Well strips)
  • SCGB1A1 Lyophilized Standard: 2
  • 100X SCGB1A1 HRP-Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 2 mL
  • Stop Solution: 1 x 20 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, tissue homogenates, cell lysates, lung lavage fluid, cell culture supernates and other biological fluids
• Sensitivity: < 0.067ng/mL

Target Info

• Gene Symbol: SCGB1A1
• Gene Full Name: secretoglobin family 1A member 1
• Alias Symbols: blastokinin;CC10;CC16;CCPBP;CCSP;clara cell phospholipid-binding protein;clara cells 10 kDa secretory protein;Secretoglobin family 1A member 1;secretoglobin, family 1A, member 1 (uteroglobin);UGB;UP1;UP-1;urinary protein 1;urine protein 1;uteroglobin.
• NCBI Gene Id: 7356
• Protein Name: Uteroglobin
• Description of Target: Binds phosphatidylcholine, phosphatidylinositol, polychlorinated biphenyls (PCB) and weakly progesterone, potent inhibitor of phospholipase A2.
• Uniprot ID: P11684
• Protein Accession #: NP_003348.1
• Nucleotide Accession #: NM_003357.4