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SCG2 ELISA Kit (Human) (OKCD02871)

96 Wells
$599.00
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Predicted Species Reactivity:
Human
Kit Detection Method:
Colorimetric, OD450 nm
Kit Duration:
3 Hours
Kit Linearity:
Sample1:21:41:81:16
Serum (n=5)80-101%87-96%88-102%91-98%
EDTA Plasma (n=5)95-105%80-94%84-92%95-103%
Heparin Plasma (n=5)99-105%78-101%78-92%95-102%
Kit Principle:
Aviva Systems Biology SCG2 ELISA Kit (Human) (OKCD02871) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for SCG2 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wellss, incubated and removed.A biotinylated detector antibody specific for SCG2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample SCG2 captured in well.
Kit Range:
1.25-80 ng/mL
Kit Recovery:
MatrixRecovery range (%)Average(%)
Serum (n=5)98-105102
EDTA Plasma (n=5)84-9893
Heparin Plasma(n=5)90-9893
Kit Reproducibility:
Intra-assay Precision: 3 samples with known low, middle and high level SCG2 was tested with 20 replicates across one plate.
Inter-assay Precision: 3 samples with known low, middle and high level SCG2 was tested on three different plates with 8 replicates, respectively.
Intra-Assay CV: <10% (n=20)
Inter-Assay CV: <12% (n=24)
Kit Component:
ComponentAmount
Anti-SCG2 Microplate96 Wells (12 x 8 Well strips)
SCG2 Lyophilized Standard2
100X Biotinylated SCG2 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
Stop Solution1 x 6 mL
TMB Substrate1 x 9 mL
Reconstitution and Storage:
Store as indicated in product manual.
Sample Type:
Serum, plasma, tissue homogenates and other biological fluids
Sensitivity:
0.49 ng/mL
Datasheets/Manuals:
Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Specificity:
This assay has high sensitivity and excellent specificity for detection of Secretogranin II (SCG2). No significant cross-reactivity or interference between Secretogranin II (SCG2) and analogues was observed.
Assay Info:
Assay Methodology: Quantitative Sandwich ELISA
Gene Symbol:
SCG2
Official Gene Full Name:
secretogranin II
Alias Symbols:
CHGC, Chromogranin-C, Secretogranin-2, Secretogranin II, SgII, SN
NCBI Gene Id:
7857
Protein Name:
Secretogranin-2
Description of Target:
Secretogranin-2 is a neuroendocrine secretory granule protein, which is the precursor for biologically active peptides.
Swissprot Id:
P13521
Protein Accession #:
NP_003460.2
Nucleotide Accession #:
NM_003469.4
Protein Size (# AA):
617
Tissue Tool:
Find tissues and cell lines supported by DNA array analysis to express SCG2.
RNA Seq:
Find tissues and cell lines supported by RNA-seq analysis to express SCG2.

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