Size:96 Wells
$419.00
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OKAG00247
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SCFR ELISA Kit (Human) : 96 Wells (OKAG00247)

Catalog#: OKAG00247
Domestic: within 1 week delivery | International: 1 week
This product is Genway GWB-SKR247
More Information
Predicted Species ReactivityHuman
Product Format1 x 96-Well Plate or 12 x 8-Well Strips
ApplicationELISA
Kit Detection MethodColorimetric, OD450 nm
Kit PrincipleThe Aviva Human SCFR ELISA Kit contains the components necessary for quantitative determination of natural or recombinant hSCFR concentrations within any experimental sample including cell lysates, serum and plasma. This particular immunoassay utilizes the quantitative technique of a "Sandwich" Enzyme-Linked Immunosorbent Assay (ELISA) where the target protein (antigen) is bound in a "sandwich" format by the primary capture antibodies coated to each well-bottom and the secondary detection antibodies added subsequently by the investigator. The capture antibodies coated to the bottom of each well are specific for a particular epitope on the Human SCFR cytokine while the user-added detection antibodies bind to epitopes on the captured target protein. Amid each step of the procedure, a series of wash steps must be performed to ensure the elimination of non-specific binding between proteins to other proteins or to the solid phase. After incubation and "sandwiching" of the target antigen, a peroxidase enzyme is conjugated to the constant heavy chain of the secondary antibody (either covalently or via Avidin/Streptavidin-Biotin interactions), allowing for a colorimetric reaction to ensue upon substrate addition. When the substrate TMB (3, 3’, 5, 5’-Tetramethylbenzidine) is added, the reaction catalyzed by peroxidase yields a blue color that is representative of the antigen concentration. Upon sufficient color development, the reaction can be terminated through addition of Stop Solution (2 N Sulfuric Acid) where the color of the solution will turn yellow. The absorbance of each well can then be read by a spectrophotometer, allowing for generation of a standard curve and subsequent determination of protein concentration.
Kit Range32-2,000 pg/mL
Kit Component
ComponentsAmount
Capture Antibody Coated Microplate96 Wells (12 x 8)
Ready-to-Use Streptavidin-HRP12 mL
Ready-to-Use Substrate12 mL
Stop Solution12 mL
Wash Buffer (15x)50 mL
Protein Standard Diluent12 mL
Sample Diluent12 mL
Detection Antibody Diluent12 mL
Biotin-Labeled Detection AntibodyLyophilized
Protein StandardLyophilized (15 ng)
Reconstitution and StorageStore as indicated in product manual.
Sample TypeCell lysates, sera and plasma
Sensitivity32 pg/mL
Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
SpecificityThe Human SCFR ELISA is capable of recognizing both recombinant and naturally produced Human SCFR proteins. The antigens listed below were tested at 50 ng/ml and did not exhibit significant cross reactivity or interference.
• Human: PDGF sRα, PDGF Rβ
• Murine: SCF R
Assay InfoQuantitative Colorimentric Sandwich ELISA
Gene SymbolKITLG
Official Gene Full NameKIT ligand
Alias Symbolsc-Kit ligand, DKFZp686F2250, FPH2, Kitl, Kit ligand, KL-1, Mast cell growth factor, MGF, SCF, SF, SHEP7, Stem cell factor
NCBI Gene Id4254
Protein NameKit ligand
Description of TargetThis gene encodes the ligand of the tyrosine-kinase receptor encoded by the KIT locus. This ligand is a pleiotropic factor that acts in utero in germ cell and neural cell development, and hematopoiesis, all believed to reflect a role in cell migration. In adults, it functions pleiotropically, while mostly noted for its continued requirement in hematopoiesis. Two transcript variants encoding different isoforms have been found for this gene.
Swissprot IdP21583
Protein Accession #NP_000890.1
Nucleotide Accession #NM_000899.4
Tissue ToolFind tissues and cell lines supported by DNA array analysis to express KITLG.
RNA SeqFind tissues and cell lines supported by RNA-seq analysis to express KITLG.
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