SCF Protein (OPPA00540)

Catalog No: OPPA00540 (Formerly GWB-F05033)

Size:2UG

Product Info

• Predicted Species Reactivity: Mouse
• Product Format: Lyophilized from a concentrated solution in water containing 0.02% NaHCO₃
  Physical Appearance: Sterile filtered white freeze-dried powder
  Protein Content: Protein quantitation was carried out by two independent methods1. UV spectroscopy at 280 nm using the absorbency value of 0.52 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using a standard solution of Stem Cell Factor as a Reference Standard.
• Reconstitution and Storage: It is recommended to reconstitute the lyophilized Stem Cell Factor in sterile 18M-omega-cm H₂O not less than 100 ug/mL, which can be further diluted to other aqueous solutions. Lyophilized KIT ligand although stable at room temperature for 3 weeks, should be stored desiccated below -18C. Upon reconstitution SCF should be stored at 4C between 2-7 days and for future use below -18C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
• Purification: Purified by proprietary chromatographic techniques.
• Purity: Greater than 98.0% as determined by (a) Analysis by SEC-HPLC (b) Analysis by SDS-PAGE
• Biological Activity: The ED₅₀ as determined by the dose-dependent stimulation of Human TF-1 cell line is <10 ng/ml, corresponding to a Specific Activity of 1x10⁵ IU/mg.
• Peptide Sequence: MKEICGNPVT DNVKDITKLV ANLPNDYMIT LNYVAGMDVL PSHCWLRDMV IQLSLSLTTL LDKFSNISEG LSNYSIIDKL GKIVDDLVLC MEENAPKNIK ESPKRPETRS FTPEEFFSIF NRSIDAFKDF MVASDTSDCV LSSTLGPEKD SRVSVTKPFM LPPVA.
• Source: E. coli

Target Info

• Gene Symbol: mSCF
• Alias Symbols: S, Gb, SC, SF, Sl, bl, Clo, Con, Kit, Mgf, SCF, SLF, Ste, blz, Kitlg, contrasted
• NCBI Gene Id: 17311
• Protein Name: Kit ligand
• Description of Target: Stem cell factor / KIT ligand (SCF) is a cytokine which binds CD117(c-Kit). SCF is also known as "steel factor" or "c-kit ligand". SCF exists in two forms, cell surface bound SCF and soluble (or free) SCF. Soluble SCF is produced by the cleavage of surface bound SCF by metalloproteases. SCF is a growth factor important for the survival, proliferation, and differentiation of hematopoietic stem cells and other hematopoietic progenitor cells. One of its roles is to change the BFU-E (burst-forming unit-erythroid) cells, which are the earliest erythrocyte precursors in the erythrocytic series, into the CFU-E (colony-forming unit-erythroid).
• Uniprot ID: P20826
• Protein Accession #: NP_038626.1
• Protein Size (# AA): Recombinant
• Molecular Weight: 18 kDa