SCF Antibody (OAPB00887)

Catalog No: OAPB00887

Size:100UG

Western blot analysis of SCF in rat brain tissue lysate with SCF antibody at (A) 1 and (B) 2 ug/mL.

Immunohistochemistry of SCF in mouse brain tissue with SCF antibody at 2.5 ug/mL.

Product Info

• Predicted Species Reactivity: Bovine|Human|Mouse|Rat
• Product Format: Liquid. SCF Antibody is supplied in PBS containing 0.02% sodium azide.
• Clonality: Polyclonal
• Host: Rabbit
• Conjugation: Unconjugated
• Application: Enzyme-linked immunosorbent assay|Immunofluorescence|Immunohistochemistry-Paraffin|Western blot
  Buffer: Antibody is supplied in PBS containing 0.02% sodium azide.
• Reconstitution and Storage: SCF antibody can be stored at 4C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Immunogen: Anti-SCF antibody (5165) was raised against a peptide corresponding to 18 amino acids near the center of human SCF. The immunogen is located within amino acids 100-150 of SCF.
• Purification: Affinity Purified
• Predicted Homology Based on Immunogen Sequence: Human; Mouse; Rat
• Concentration: 1 mg/ml
• Blocking Peptide: For anti-SCF antibody is Catalog # OEPB03009
• Application Info: SCF antibody can be used for detection of SCF by Western blot at 1 - 2 ug/ml. (Optimal dilution should be determined by user)
• Reference: Reber L, Da Silva CA, and Frossard N. Stem cell factor and its receptor c-Kit as targets for inflammatory diseases. Euro. J. Pharmacology2006; 533:327-40.;Jensen BM, Metcalfe DD, and Gilfillan AM. Targeting kit activation: a potential therapeutic approach in the treatment of allergic inflammation. Inflamm. Allergy Drug Targets2007; 6:57-62.;Lukacs NW, Strieter RM, Lincoln PM, et al. Stem cell factor (c-kit ligand) influences eosinophil recruitment and histamine levels in allergic airway inflammation. J. Immunol.1996; 156:3945-51.;

Target Info

• Gene Symbol: KITLG
• Gene Full Name: KIT ligand
• Alias Symbols: c-Kit ligand;DCUA;DFNA69;familial progressive hyperpigmentation 2;FPH2;FPHH;kit ligand;Kitl;KL-1;mast cell growth factor;MGF;SCF;SF;SHEP7;SLF;steel factor;stem cell factor.
• NCBI Gene Id: 4254
• Protein Name: Kit ligand
• Description of Target: Ligand for the receptor-type protein-tyrosine kinase KIT. Plays an essential role in the regulation of cell survival and proliferation, hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, migration and function, and in melanogenesis. KITLG/SCF binding can activate several signaling pathways. Promotes phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, and subsequent activation of the kinase AKT1. KITLG/SCF and KIT also transmit signals via GRB2 and activation of RAS, RAF1 and the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1. KITLG/SCF and KIT promote activation of STAT family members STAT1, STAT3 and STAT5. KITLG/SCF and KIT promote activation of PLCG1, leading to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate. KITLG/SCF acts synergistically with other cytokines, probably interleukins.
• Uniprot ID: P21583
• Molecular Weight: Predicted: 31kD Observed: 36 kD (31kD + 4 N-linked Glycosylations)