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Catalog No: OKAG00060 (Formerly GWB-SKR060)
Size:12x8WELLS
Price: $475.00
SKU
OKAG00060
Availability: Domestic: within 1-2 week delivery | International: 1-2 weeks
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHomo sapiens, Human
Product Format1 x 96-Well Plate or 12 x 8-Well Strips
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric 450 nm
ELISA Kit Duration4.5 hours
ELISA Kit PrincipleThe Aviva Human sCD40 Ligand ELISA Kit contains the components necessary for quantitative determination of natural or recombinant hsCD40 Ligand concentrations within any experimental sample including cell lysates, serum and plasma. This particular immunoassay utilizes the quantitative technique of a "Sandwich" Enzyme-Linked Immunosorbent Assay (ELISA) where the target protein (antigen) is bound in a "sandwich" format by the primary capture antibodies coated to each well-bottom and the secondary detection antibodies added subsequently by the investigator. The capture antibodies coated to the bottom of each well are specific for a particular epitope on the Human sCD40 Ligand cytokine while the user-added detection antibodies bind to epitopes on the captured target protein. Amid each step of the procedure, a series of wash steps must be performed to ensure the elimination of non-specific binding between proteins to other proteins or to the solid phase. After incubation and "sandwiching" of the target antigen, a peroxidase enzyme is conjugated to the constant heavy chain of the secondary antibody (either covalently or via Avidin/Streptavidin-Biotin interactions), allowing for a colorimetric reaction to ensue upon substrate addition. When the substrate TMB (3, 3’, 5, 5’-Tetramethylbenzidine) is added, the reaction catalyzed by peroxidase yields a blue color that is representative of the antigen concentration. Upon sufficient color development, the reaction can be terminated through addition of Stop Solution (2 N Sulfuric Acid) where the color of the solution will turn yellow. The absorbance of each well can then be read by a spectrophotometer, allowing for generation of a standard curve and subsequent determination of protein concentration.
ELISA Kit Range32-2000 pg/ml
ELISA Kit Component
ComponentsAmount
Capture Antibody Coated Microplate96 Wells (12 x 8)
Ready-to-Use Streptavidin-HRP12 mL
Ready-to-Use Substrate12 mL
Stop Solution12 mL
Wash Buffer (15x)50 mL
Protein Standard Diluent12 mL
Sample Diluent12 mL
Detection Antibody Diluent12 mL
Biotin-Labeled Detection AntibodyLyophilized
Protein StandardLyophilized (100 ng)
Reconstitution and Storage2°C to 8°C
Sample TypeCell lysates, sera and plasma
Sensitivity32 pg/mL
SpecificityThe Human sCD40 Ligand ELISA is capable of recognizing both recombinant and naturally produced Human sCD40 Ligand proteins. The antigens listed below were tested at 50 ng/ml and exhibited less than 1% cross reactivity.
• Murine: sCD40L
The antigens listed below were tested at 50 ng/ml and did not exhibit significant cross reactivity or interference.
• Human: 4-1BBL, BAFF, sFasL, FasR, IL-1α, sRANKL, sRANKR, sTNF-RI, sTNF-RII, TNFα, TNFβ, sTRAIL, sTRAIL-RI, sTRAIL-RII, TWEAK
• Murine: sRANKL, TNFα
• Rat: TNFα
Assay InfoQuantitative Colorimentric Sandwich ELISA
Gene SymbolCD40LG
Gene Full NameCD40 ligand
Alias SymbolsCD154;CD40 antigen ligand;CD40 ligand;CD40L;CD40-L;gp39;hCD40L;HIGM1;IGM;IMD3;T-B cell-activating molecule;T-BAM;T-cell antigen Gp39;TNF-related activation protein;TNFSF5;TRAP;tumor necrosis factor (ligand) superfamily member 5;Tumor necrosis factor ligand superfamily member 5.
NCBI Gene Id959
Protein NameP29965
Description of TargetCD40 ligand
Uniprot IDhttps://www.uniprot.org/uniprot/P29965
Protein Accession #NP_000065.1
Nucleotide Accession #NM_000074.2
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