Catalog No: OKEH07143
Size:96T
Price: $600.00
SKU
OKEH07143
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHomo sapiens, Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD 450 nm
ELISA Kit Duration~3 Hours
ELISA Kit PrincipleAviva Systems Biology SCD ELISA Kit (Human) (OKEH07143) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for SCD has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for SCD is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample SCD captured in the well.
ELISA Kit Range0.156-10ng/mL
ELISA Kit RecoveryMean recovery when spiking into matrix = 103%
ELISA Kit ReproducibilityIntra-assay reproducibility was evaluated with 20 replicates of 3 samples representing low, middle and high level LIPC. Inter-assay reproducibility was evaluated with 3 samples representing low, middle and high level LIPC using 8 replicates on each of 3 plates.
Intra-Assay CV <5.7%
Inter-Assay CV <7.0%
ELISA Kit Component
ComponentAmount
SCD Microplate96 Wells (12 x 8 Well strips)
SCD Lyophilized Standard2 x 10 ng
100X SCD HRP-Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
100X Luminol Substrate1 x 2 mL
Substrate Diluent1 x 20 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates
Sensitivity0.078 ng/mL
SpecificityNatural and recombinant Human Acyl-CoA desaturase
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolSCD
Gene Full Namestearoyl-CoA desaturase
Alias Symbolsacyl-CoA desaturase;delta(9)-desaturase;FADS5;fatty acid desaturase;hSCD1;MSTP008;predicted protein of HQ0998;SCD1;SCDOS;stearoyl-CoA desaturase;stearoyl-CoA desaturase (delta-9-desaturase);stearoyl-CoA desaturase opposite strand.
NCBI Gene Id6319
Protein NameO00767
Description of TargetAcyl-CoA desaturase
Uniprot IDhttps://www.uniprot.org/uniprot/O00767
Protein Accession #NP_005054
Nucleotide Accession #NM_005063
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