SCD Antibody (OAEB00850)

Catalog No: OAEB00850 (Formerly GWB-9EBAAE)

Size:100UG

OAEB00850 (5ug/ml) staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Product Info

• Predicted Species Reactivity: Human
• Product Format: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Clonality: Polyclonal
• Host: Goat
• Application: IHC
• Additional Information: Epitope Info: C Terminus
  Immunogen: Peptide with sequence C-RIKRTGDGNYKSG, from the C Terminus of the protein sequence according to NP_005054.3.
  Reactivity Info: Tested: Human; Expected: Human
• Reconstitution and Storage: Aliquot and store at -20C. Minimize freezing and thawing.
• Immunogen: SCD
• Purification: Ammonium Sulfate Precipitated; Affinity Purified
• Predicted Homology Based on Immunogen Sequence: Cow: 76%; Dog: 92%; Guinea Pig: 83%; Human: 100%; Mouse: 84%; Pig: 84%; Zebrafish: 76%
• Peptide Sequence: C-RIKRTGDGNYKSG
• Concentration: 0.5 mg/ml
• Purity: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Application Info: Peptide ELISA: Antibody detection limit dilution 1:64000.
  Immunohistochemistry: In paraffin embedded Human Liver shows textured cytoplam staining in hepatocytes. Recommended concentration, 5-10ug/ml.
  Western Blot: Preliminary experiments gave an approx 30kDa band in Human Adipose, Brain and Liver lysates after 0.3 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 41.5kDa according to NP_005054.3. The 30kDa band was successfully blocked by incubation with the immunizing peptide.
• Reference: Zhang S, Yang Y, Shi Y.
  Characterization of human SCD2, an oligomeric desaturase with improved stability and enzyme activity by cross-linking in intact cells.
  Biochem J. 2004 Dec 20; [Epub ahead of print] PMID: 15610069

Target Info

• Gene Symbol: SCD
• Gene Full Name: stearoyl-CoA desaturase
• Alias Symbols: acyl-CoA desaturase;delta(9)-desaturase;FADS5;fatty acid desaturase;hSCD1;MSTP008;predicted protein of HQ0998;SCD1;SCDOS;stearoyl-CoA desaturase;stearoyl-CoA desaturase (delta-9-desaturase);stearoyl-CoA desaturase opposite strand.
• NCBI Gene Id: 6319
• Protein Name: Acyl-CoA desaturase
• Uniprot ID: O00767
• Protein Accession #: NP_005054.3
• Molecular Weight: 41.5