SCARB1 ELISA Kit (Mouse) (OKCD02868)

Catalog No: OKCD02868

Size:96WELLS

Mouse SCARB1 ELISA kit standard curve

Product Info

• Predicted Species Reactivity: Mouse
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colormetric|Regular detection
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2	1:4	1:8	1:16
  Serum (n=5)	80-94%	91-98%	95-102%	90-104%
  EDTA Plasma (n=5)	79-95%	80-89%	95-102%	82-97%
  Heparin Plasma (n=5)	95-105%	80-95%	92-105%	84-101%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Scavenger Receptor Class B Member 1 (SCARB1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Scavenger Receptor Class B Member 1 (SCARB1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Scavenger Receptor Class B Member 1 (SCARB1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Scavenger Receptor Class B Member 1 (SCARB1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Recovery:

  Matrix	Recovery range (%)	Average(%)
  Serum (n=5)	95-102	98
  EDTA Plasma (n=5)	94-101	98
  Heparin Plasma(n=5)	81-98	88

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Scavenger Receptor Class B Member 1 (SCARB1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Scavenger Receptor Class B Member 1 (SCARB1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-SCARB1 Microplate: 96 Wells (12 x 8 Well strips)
  • SCARB1 Lyophilized Standard: 2
  • 100X Biotinylated SCARB1 Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • Stop Solution: 1 x 6 mL
  • TMB Substrate: 1 x 9 mL
• Reconstitution and Storage: 2°C to 8°C, -20°C
• Sample Type: Serum, plasma, tissue homogenates, cell lysates and other biological fluids
• Sensitivity: 0.068 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Scavenger Receptor Class B Member 1 (SCARB1). No significant cross-reactivity or interference between Scavenger Receptor Class B Member 1 (SCARB1) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: SCARB1
• Gene Full Name: scavenger receptor class B, member 1
• Alias Symbols: S, Sr, Cla, CD36, Cla1, Hdlq, SRBI, Srb1, Cd36l, Chohd, Cla-1, Hdlq1, Hlb39, SR-B1, SR-BI, Cd36l1, Chohd1, Hlb398, mSR-BI, AI120173, D5Ertd460, D5Ertd460e
• NCBI Gene Id: 20778
• Protein Name: Scavenger receptor class B member 1
• Description of Target: Receptor for different ligands such as phospholipids, cholesterol ester, lipoproteins, phosphatidylserine and apoptotic cells. Probable receptor for HDL, located in particular region of the plasma membrane, called caveolae. Facilitates the flux of free and esterified cholesterol between the cell surface and extracellular donors and acceptors, such as HDL and to a lesser extent, apoB-containing lipoproteins and modified lipoproteins. Probably involved in the phagocytosis of apoptotic cells, via its phosphatidylserine binding activity. Plays an important role in the uptake of HDL cholesteryl ester.
• Uniprot ID: Q61009
• Protein Accession #: NP_001192011.1
• Nucleotide Accession #: NM_001205082.1
• Protein Size (# AA): 509