Aviva Systems Biology will be closed in observance of Good Friday 3/29/2024. If you are in need of any assistance please email info@avivasysbio.com

Catalog No: OKCD01947 (Formerly GWB-KBBMJ4)
Size:96WELLS
Price: $700.00
SKU
OKCD01947
Availability: Domestic: within 1-2 weeks delivery | International: within 1-2 weeks delivery
Please contact customer service when ordering 10+ kits.

Bulk
Order
Aviva's
Satisfaction
Guarantee
Contact Us:
Shipping Info:
  • $55: Antibody & Protein in US
  • $55 + $25/Kit in US
  • Contact us for international orders.
Datasheets/ManualsPrintable datasheet for SCARA3 ELISA kit (Mouse)
Product Info
Predicted Species ReactivityMouse
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColormetric|Regular detection
ELISA Kit Duration3h
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Scavenger Receptor Class A Member 3 (SCARA3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Scavenger Receptor Class A Member 3 (SCARA3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Scavenger Receptor Class A Member 3 (SCARA3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Scavenger Receptor Class A Member 3 (SCARA3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.781-50ng/mL
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Scavenger Receptor Class A Member 3 (SCARA3) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Scavenger Receptor Class A Member 3 (SCARA3) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-Scara3 Microplate96 Wells (12 x 8 Well Strips)
Scara3 Lyophilized Standard2 x 50 ng
100X Biotinylated Scara3 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C, -20°C
Sample TypeTissue homogenates and other biological fluids
Sensitivity< 0.33 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Scavenger Receptor Class A Member 3 (SCARA3). No significant cross-reactivity or interference between Scavenger Receptor Class A Member 3 (SCARA3) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
Gene SymbolSCARA3
Gene Full NameScavenger receptor class A, member 3
Alias SymbolsCSR, APC7, CSR1, MSLR1, MSRL1, C130058N24Rik
NCBI Gene Id219151
Protein NameScavenger receptor class A member 3
Description of TargetSeems to protect cells by scavenging oxidative molecules or harmful products of oxidation.
Uniprot IDQ8C850
Protein Accession #NP_766192.1
Nucleotide Accession #NM_172604.3
Protein Size (# AA)606
Write Your Own Review
You're reviewing:Scara3 ELISA Kit (Mouse) (OKCD01947)
Your Rating
We found other products you might like!