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SBA Clonotyping System- HRP Kit (OKSB00003)

1 Kit
$565.00
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Application Kit:
Horseradish peroxidase (HRP)- based Enzyme- Linked- Immunosorbent- Assay(ELISA) and immunoblotting
- 1 mL goat anti- mouse Ig capture antibody (2.5 mg/mL)
- 1 mL HRP- labeled goat anti- mouse Ig screening antibody
- 1 mL HRP- labeled goat anti- mouse IgM
- 1 mL HRP- labeled goat anti- mouse IgG1
- 1 mL HRP- labeled goat anti- mouse IgG2a
- 1 mL HRP- labeled goat anti- mouse IgG2b
- 1 mL HRP- labeled goat anti- mouse IgG3
- 1 mL HRP- labeled goat anti- mouse IgA
- 1 mL HRP- labeled goat anti- mouse κ
- 1 mL HRP- labeled goat anti- mouse λ
- ABTS substrate (100 mg)
Datasheets/Manuals:
Printable datasheet for OKSB00003
Product Format:
Kit
Application Info:
- Initial screening of fusions and selection of antibody-producing hybridomas
- Identification of the heavy and light chain isotypes of antibodies
- Selection and isolation of isotype switch variants
Storage:
- The purified (UNLB) antibody is supplied 100 mM borate buffered saline, pH 8.2. No preservatives oramine- containing buffer salts added. Store at 2- 8 C
- The horseradish peroxidase (HRP) conjugates are supplied in a stock solution in 50% glycerol/50%PBS, pH 7.4. No preservative added. Store at 2- 8 C
- All system components can be stored for several months at 2- 8 C without appreciable loss of activity
For long- term storage, the purified antibody and HRP conjugates should be aliquoted undiluted andstores at - 20 C.
Characterization:
To ensure lot- to- lot consistency, each batch of product is tested by ELISA for conformance tocharacteristics of a standard reference reagent.
Dilution:
ELISA (HRP): 1:250-1:500
Immunoblotting: Since immunoblotting methods vary, you should determine the optimum
working dilution of the product that is appropriate for your specific need.

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