SBA Clonotyping System Kit : HRP (OKSB00003)

Catalog No: OKSB00003

Size:1Kit

Product Info

• Tested Species Reactivity: Mouse
• Clonality: Polyclonal
• Host: Goat
• Conjugation: HRP: Horseradish Peroxidase
• Application: ELISA
• Reconstitution and Storage: Store at 2-8C
• Application Kit: Horseradish peroxidase (HRP)- based Enzyme- Linked- Immunosorbent- Assay(ELISA) and immunoblotting
  - 1 mL goat anti- mouse Ig capture antibody (2.5 mg/mL)
  - 1 mL HRP- labeled goat anti- mouse Ig screening antibody
  - 1 mL HRP- labeled goat anti- mouse IgM
  - 1 mL HRP- labeled goat anti- mouse IgG1
  - 1 mL HRP- labeled goat anti- mouse IgG2a
  - 1 mL HRP- labeled goat anti- mouse IgG2b
  - 1 mL HRP- labeled goat anti- mouse IgG3
  - 1 mL HRP- labeled goat anti- mouse IgA
  - 1 mL HRP- labeled goat anti- mouse κ
  - 1 mL HRP- labeled goat anti- mouse λ
  - ABTS substrate (100 mg)
• Application Info: Initial screening of fusions and selection of antibody-producing hybridomas, Identification of the heavy and light chain isotypes of antibodies, Selection and isolation of isotype switch variants
• Storage: - The purified (UNLB) antibody is supplied 100 mM borate buffered saline, pH 8.2. No preservatives oramine- containing buffer salts added. Store at 2- 8 C
  - The horseradish peroxidase (HRP) conjugates are supplied in a stock solution in 50% glycerol/50%PBS, pH 7.4. No preservative added. Store at 2- 8 C
  - All system components can be stored for several months at 2- 8 C without appreciable loss of activity
  For long- term storage, the purified antibody and HRP conjugates should be aliquoted undiluted andstores at - 20 C.
• Characterization: To ensure lot- to- lot consistency, each batch of product is tested by ELISA for conformance tocharacteristics of a standard reference reagent.
• Dilution: ELISA (HRP): 1:250-1:500
  Immunoblotting: Since immunoblotting methods vary, you should determine the optimum
  working dilution of the product that is appropriate for your specific need.