SARS1 ELISA Kit (Human) (OKEH08296)

Catalog No: OKEH08296

Size:96T

Human SARS1 ELISA Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens, Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Principle: Aviva Systems Biology SARS1 ELISA Kit (Human) (OKEH08296) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for SARS1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for SARS1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample SARS1 captured in well.
• ELISA Kit Range: 31.2-2000pg/mL
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 98% (n = 20)
• ELISA Kit Reproducibility: Mean Intra-assay CV%: <=2.5% (n = 20)
  Mean Inter-assay CV%: <=4.8% (n = 20)
• ELISA Kit Component:
  • SARS1 Microplate: 96 Wells (12 x 8 Well strips)
  • SARS1 Lyophilized Standard: 2
  • 100X Biotinylated SARS1 Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Additional Information: Function: Catalyzes the attachment of serine to tRNA(Ser). Is also probably able to aminoacylate tRNA(Sec) with serine, to form the misacylated tRNA L-seryl-tRNA(Sec), which will be further converted into selenocysteinyl-tRNA(Sec).
  Subcellular Location: Cytoplasm
  Unigene: Hs.531176
  SMR: P49591
  String: 9606.ENSP00000234677
  KEGG: hsa:6301
  MIM: 607529
  Pfam: PF02403
• Reconstitution and Storage: Store as indicated in product manual.
• Sensitivity: 14.9pg/mL
• Specificity: Natural and recombinant Human Serine--tRNA ligase, cytoplasmic
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: SARS1
• Gene Full Name: seryl-tRNA synthetase 1
• Alias Symbols: NEDMAS;SARS;serine tRNA ligase 1, cytoplasmic;serine--tRNA ligase, cytoplasmic;SERRS;SERS;Seryl-tRNA synthetase;seryl-tRNA synthetase, cytoplasmic;seryl-tRNA(Ser/Sec) synthetase.
• NCBI Gene Id: 6301
• Protein Name: P49591
• Description of Target: Serine--tRNA ligase, cytoplasmic
• Uniprot ID: https://www.uniprot.org/uniprot/P49591
• Protein Accession #: NP_006504.2
• Nucleotide Accession #: NM_006513.3