SARS-CoV-2 Spike RBD E484K, N501Y (South Africa Variant Beta) Recombinant Protein (OPSN00006)

Catalog No: OPSN00006

Size:100ug

Purified protein (1 ug) was loaded onto a 4-12% Bis-tris SDS-PAGE gel, electrophoresed and coomassie stained to demonstrate recombinant protein integrity and purity. Gels were imaged with a white background post destain.

Product Info

• Predicted Species Reactivity: SARS-CoV-2
• Product Format: Liquid. 50 mM Tris-HCL, 1M NaCl, pH 7.5
• Host: Sf9 Baculovirus
• Conjugation: Unconjugated
• Application: IA, AA, FN
• Additional Information: Lineage: B.1.351, 20H/501Y.V2
• Reconstitution and Storage: Store at -20C
• Formulation: 50 mM Tris-HCL, 1M NaCl, pH 7.5
• Purification: IMAC
• Concentration: 0.14 mg/mL
• Purity: 90%
• Tag: C-Avitag/His
• Reference: Emergence and rapid spread of a new severe acute respiratory syndrome-related coronavirus 2 (SARS-CoV-2) lineage with multiple spike mutations in South Africa https://doi.org/10.1101/2020.12.21.20248640. Sci Rep. 2021 Apr 28;11(1):9166. doi: 10.1038/s41598-021-88696-5.
• Description: The SARS-CoV-2 Spike Receptor Binding Domain E484K, N501Y (South Africa Variant Beta) Recombinant Protein (OPSN00006) is expressed in Sf9 Baculovirus cells and includes a C-terminal Avidin-His Tag with purification via IMAC. The sequence includes amino acids 319-357 of the lineage B.1.351, 20H/501Y.V2 SARS-CoV-2 spike sequence first identified in South Africa. Suitable for Biotinylation and Protein:Protein Interaction assays in a variety of formats.

Target Info

• Gene Symbol: S (S1)
• Gene Full Name: surface glycoprotein
• Alias Symbols: spike glycoprotein
• NCBI Gene Id: 43740568
• Protein Name: surface glycoprotein
• Description of Target: Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is an enveloped, positive-sense, single-stranded RNA virus that causes coronavirus disease 2019 (COVID-19). Virus particles include the RNA genetic material and structural proteins needed for invasion of host cells. Once inside the cell the infecting RNA is used to encode structural proteins that make up virus particles, nonstructural proteins that direct virus assembly, transcription, replication and host control and accessory proteins whose function has not been determined.~ The structural proteins of SARS-CoV-2 include the envelope protein (E), spike or surface glycoprotein (S), membrane protein (M) and the nucleocapsid protein (N). The spike glycoprotein is found on the outside of the virus particle and gives coronavirus viruses their crown-like appearance. This glycoprotein mediates attachment of the virus particle and entry into the host cell. S protein is an important target for vaccine development, antibody therapies and diagnostic antigen-based tests.
• Uniprot ID: P0DTC2
• Protein Size (# AA): AA 319-537, E484K, N501Y (South Africa Variant Beta)
• Molecular Weight: 30kDa