SARS-CoV-2 Envelope Antibody (OAPB00317)

Catalog No: OAPB00317

Size:100UG

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Figure 1 ELISA Test
Antibodies: SARS-CoV-2 (COVID-19, 2019-nCoV) Envelope Antibody (1 ug/mL). A sandwich ELISA was performed using antigen or control peptide as coating antigen and the anti-SARS-CoV-2 (COVID-19, 2019-nCoV) Envelope antibody as the capture antibody. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:20000 dilution. Detection range is from 32 ng/mL to 2000ng/mL.

Product Info

• Predicted Species Reactivity: Severe Acute Respiratory Syndrome Coronavirus|Virus
• Product Format: Liquid. SARS-CoV-2 (COVID-19, 2019-nCoV) Envelope Antibody is supplied in PBS containing 0.02% sodium azide.
• Clonality: Polyclonal
• Isotype: IgG
• Host: Rabbit
• Conjugation: Unconjugated
• Application: Enzyme-linked immunosorbent assay|Immunofluorescence|Immunohistochemistry
• Reconstitution and Storage: Stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Immunogen: Anti-SARS-CoV-2 (COVID-19, 2019-nCoV) Envelope antibody (3531) was raised against a peptide corresponding to 10 amino acids near the amino terminus of SARS-CoV-2 (COVID-19, 2019-nCoV) Envelope protein. The immunogen is located within the first 50 amino acids of SARS-CoV-2 (COVID-19, 2019-nCoV) Envelope.
• Purification: Affinity Purified
• Predicted Homology Based on Immunogen Sequence: SARS-CoV Envelope proteins: (100%)
• Concentration: 1 mg/ml
• Blocking Peptide: For anti-SARS Envelope antibody is Catalog # OEPB02410
• Application Info: IHC/IF: 2-3 ug/mL
  Antibody validated in IHC and IF in COVID-19 patient samples. SARS-CoV-2 (COVID-19, 2019-nCoV) Envelope antibody can detect 50 ng of free peptide at 1 ug/mL in ELISA. But it cannot detect envelope recombinant protein in WB and ELISA.
• Reference: Gorbalenya. bioRxiv: 2020.
  Hui et al. Int J Infect Dis. 2020;91:264-266.
  Pervushin et al. PLoS Pathog. 2009; 5(7): e1000511.

Target Info

• Gene Symbol: E
• Gene Full Name: envelope protein
• Alias Symbols: envelope protein;GU280_gp04.
• NCBI Gene Id: 43740570
• Protein Name: envelope protein [Severe acute respiratory syndrome coronavirus 2]
• Description of Target: Coronavirus disease 2019 (COVID-19), formerly known as 2019-nCoV acute respiratory disease, is an infectious disease caused by SARS-CoV-2, a virus closely related to the SARS virus. The disease is the cause of the 2019–20 coronavirus outbreak. The structure of 2019-nCoV consists of the following: a spike protein (S), hemagglutinin-esterease dimer (HE), a membrane glycoprotein (M), an envelope protein (E) a nucleoclapid protein (N) and RNA. Envelope protein is a small polypeptide that contains at least one alpha-helical transmembrane domain. It involves in several aspects of the virus's life cycle, such as assembly, budding, envelope formation, and pathogenesis. E protein has membrane permeabilizing activity, which provides a possible rationale to inhibit in vitro ion channel activity of some synthetic coronavirus E proteins, and also viral replication.
• Protein Accession #: https://www.ncbi.nlm.nih.gov/protein/QHD43418