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Catalog No: OKEI00275
Size:96 Wells
Price: $625.00
SKU
OKEI00275
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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  • $55: Antibody & Protein in US
  • $55 + $25/Kit in US
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHuman
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology SAP25 ELISA Kit (Human) (OKEI00275) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for SAP25 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for SAP25 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of a luminol substrate which is catalyzed by the HRP to produce light emission. The light emission is read by a luminometer (or photo-multiplier equipped instrument) and the intensity of the emitted light is proportional to the amount of sample SAP25
ELISA Kit Range0.031 - 2 ng/mL
ELISA Kit ReproducibilityIntra-assay reproducibility was evaluated using 20 replicates of 3 samples representing low, middle and high level SAP25. Inter-assay reproducibility was evaluated with 3 samples representing low, middle and high level SAP25 using 8 replicates on each of 3 plates.
Intra-Assay CV <10%
Inter-Assay CV <12%
ELISA Kit Component
ComponentAmount
SAP25 Microplate96 Wells (12 x 8 Well strips)
SAP25 Lyophilized Standard2
100X SAP25 HRP-Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 10 mL
Conjugate Diluent1 x 10 mL
30X Wash Buffer1 x 30 mL
100X Luminol Substrate1 x 10 mL
Substrate Diluent1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Biological Fluids
Sensitivity18.75 pg/mL
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolSAP25
NCBI Gene Id100316904
Protein NameHistone deacetylase complex subunit SAP25
Description of TargetInvolved in the transcriptional repression mediated by the mSIN3A but not the N-CoR corepressor complex.By similarity
Uniprot IDQ8TEE9
Protein Accession #NP_001335606
Nucleotide Accession #NM_001348677
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