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Catalog No: OKCD04464
Size:96WELLS
Price: $450.00
SKU
OKCD04464
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Datasheets/ManualsPrintable datasheet for SAP ELISA Kit (Mouse) (OKCD04464)
Product Info
Predicted Species ReactivityMouse|Mus musculus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit Linearity
Sample1:21:41:81:16
Serum (n=5)85-95%79-90%99-105%98-105%
EDTA Plasma (n=5)81-101%84-91%87-94%87-94%
Heparin Plasma (n=5)84-98%87-104%98-105%78-92%
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Serum Amyloid P Component (SAP). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Serum Amyloid P Component (SAP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Serum Amyloid P Component (SAP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Serum Amyloid P Component (SAP) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range3.12-200ng/mL
ELISA Kit Recovery
MatrixRecovery range (%)Average (%)
Serum (n=5)80-9285
EDTA Plasma (n=5)80-9384
Heparin Plasma (n=5)91-9895
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Serum Amyloid P Component (SAP) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Serum Amyloid P Component (SAP) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Ltb Microplate96 Wells (12 x 8 Well strips)
Ltb Lyophilized Standard2
100X Ltb HRP-Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma, tissue homogenates and other biological fluids
Sensitivity1.37 ng/mL
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolApcs
Gene Full Nameamyloid P component, serum
Alias SymbolsAPCS amyloid P-component serum protein;Sap;serum amyloid P-component.
NCBI Gene Id20219
Protein NameSerum amyloid P-component
Description of TargetSerum amyloid P-component
Uniprot IDP12246
Protein Accession #NP_035448.2
Nucleotide Accession #NM_011318.2
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