SAMHD1 ELISA Kit (Human) (OKEH08554)

Catalog No: OKEH08554

Size:96T

Product Info

• Predicted Species Reactivity: Homo sapiens, Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Principle: Aviva Systems Biology SAMHD1 ELISA Kit (Human) (OKEH08554) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for SAMHD1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for SAMHD1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample SAMHD1 captured in well.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 110% (n = 20)
• ELISA Kit Reproducibility: Mean Intra-assay CV%: <=7.6% (n = 20)
  Mean Inter-assay CV%: <=11.9% (n = 20)
• ELISA Kit Component:
  • SAMHD1 Microplate: 96 Wells (12 x 8 Well strips)
  • SAMHD1 Lyophilized Standard: 2
  • 100X Biotinylated SAMHD1 Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Additional Information: Function: Protein that acts both as a host restriction factor involved in defense response to virus and as a regulator of DNA end resection at stalled replication forks (PubMed:19525956, PubMed:21613998, PubMed:21720370, PubMed:23602554, PubMed:23601106, PubMed:22056990, PubMed:24336198, PubMed:26294762, PubMed:26431200, PubMed:28229507, PubMed:28834754, PubMed:29670289). Has deoxynucleoside triphosphate (dNTPase) activity, which is required to restrict infection by viruses, such as HIV-1: dNTPase activity reduces cellular dNTP levels to levels too low for retroviral reverse transcription to occur, blocking early-stage virus replication in dendritic and other myeloid cells (PubMed:19525956, PubMed:21613998, PubMed:21720370, PubMed:23602554, PubMed:23601106, PubMed:23364794, PubMed:25038827, PubMed:26101257, PubMed:22056990, PubMed:24336198, PubMed:28229507, PubMed:26294762, PubMed:26431200). Likewise, suppresses LINE-1 retrotransposon activity (PubMed:24035396, PubMed:29610582, PubMed:24217394). Not able to restrict infection by HIV-2 virus; because restriction activity is counteracted by HIV-2 viral protein Vpx (PubMed:21613998, PubMed:21720370). In addition to virus restriction, dNTPase activity acts as a regulator of DNA precursor pools by regulating dNTP pools (PubMed:23858451). Phosphorylation at Thr-592 acts as a switch to control dNTPase-dependent and -independent functions: it inhibits dNTPase activity and ability to restrict infection by viruses, while it promotes DNA end resection at stalled replication forks (PubMed:23602554, PubMed:23601106, PubMed:29610582, PubMed:29670289). Functions during S phase at stalled DNA replication forks to promote the resection of gapped or reversed forks: acts by stimulating the exonuclease activity of MRE11, activating the ATR-CHK1 pathway and allowing the forks to restart replication (PubMed:29670289). Its ability to promote degradation of nascent DNA at stalled replication forks is required to prevent induction of type I interferons, thereby preventing chronic inflammation (PubMed:27477283, PubMed:29670289). Ability to promote DNA end resection at stalled replication forks is independent of dNTPase activity (PubMed:29670289). Enhances immunoglobulin hypermutation in B-lymphocytes by promoting transversion mutation (By similarity).
  Subcellular Location: Nucleus Chromosome Localizes to sites of DNA double-strand breaks in response to DNA damage.
  Unigene: Hs.580681
  SMR: Q9Y3Z3
  String: 9606.ENSP00000262878
  KEGG: hsa:25939
  MIM: 606754
  Pfam: PF01966
• Reconstitution and Storage: Store as indicated in product manual.
• Sensitivity: 0.078ng/mL
• Specificity: Natural and recombinant Human Deoxynucleoside triphosphate triphosphohydrolase SAMHD1
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: SAMHD1
• Gene Full Name: SAM and HD domain containing deoxynucleoside triphosphate triphosphohydrolase 1
• Alias Symbols: CHBL2;DCIP;dendritic cell-derived IFNG-induced protein;deoxynucleoside triphosphate triphosphohydrolase SAMHD1;dNTPase;HDDC1;hSAMHD1;monocyte protein 5;MOP-5;SAM domain and HD domain 1;SAM domain and HD domain-containing protein 1;SBBI88.
• NCBI Gene Id: 25939
• Protein Name: Q9Y3Z3
• Description of Target: Deoxynucleoside triphosphate triphosphohydrolase SAMHD1
• Uniprot ID: https://www.uniprot.org/uniprot/Q9Y3Z3
• Protein Accession #: NP_056289.2
• Nucleotide Accession #: NM_015474.3