Catalog No: OKEH03372
Size:96T
Price: $600.00
SKU
OKEH03372
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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  • $55: Antibody & Protein in US
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Please click here to view the MSDS.
Product Info
Predicted Species ReactivityHomo sapiens, Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology SAA2 ELISA Kit (Human) (OKEH03372) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for SAA2 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for SAA2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample SAA2 captured in well.
ELISA Kit Range31.2-2000pg/mL
ELISA Kit ReproducibilityMean Intra-assay CV%: < 5.8% (n = 20)
Mean Inter-assay CV%: < 9.4% (n = 20)
ELISA Kit Component
ComponentAmount
Anti-SAA2 Microplate96 Wells (12 x 8 Well strips)
SAA2 Lyophilized Standard2
Sample Diluent1 x 20 mL
100X Biotinylated SAA2 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Urine, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Sensitivity15.6 pg/mL
SpecificityNatural and recombinant Human Serum amyloid A-2 protein
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolSAA2
Gene Full Nameserum amyloid A2
Alias SymbolsSAA;SAA1;Serum amyloid A-1 protein;serum amyloid A-2 protein.
NCBI Gene Id6289
Protein NameP0DJI9
Description of TargetSerum amyloid A-2 protein
Uniprot IDhttps://www.uniprot.org/uniprot/P0DJI9
Protein Accession #NP_001120852.1
Nucleotide Accession #NM_001127380.2
Protein Size (# AA)122
Molecular Weight14 kDa
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