SAA ELISA Kit (Mouse) (OKBB00384)

Catalog No: OKBB00384

Size:96 Wells

Mouse SAA ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Mouse
• Application: ELISA
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: 2.5 Hours
• ELISA Kit Principle: Aviva's mouse SAA ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A polyclonal antibody from goat specific for SAA has been precoated onto 96-well plates. Standards(E.coli, G20-Y122) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for SAA is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the mouse SAA amount of sample captured in plate.
• ELISA Kit Range: 1.56 ng/ml - 100 ng/ml
• ELISA Kit Reproducibility:

  	Intra-Assay Precision			Inter-Assay Precision
  Sample	1	2	3	1	2	3
  n	16	16	16	24	24	24
  Mean (pg/mL)	1.4	3.4	5.7	1.5	3.6	6.8
  SD	0.06	0.177	0.291	0.081	0.223	0.388
  CV%	4.3	5.2	5.1	5.4	6.2	5.7

• ELISA Kit Component:
  • 96-Well plate Pre-coated with Anti-Mouse SAA Antibody: 1 x (12 x 8 Well Strip)
  • Lyophilized Recombinant Mouse SAA Standard: 2 x 10 ng/tube
  • 100X Biotinylated Anti-Mouse SAA Antibody: 1 x 130 uL
  • 100X Avidin-Biotin-Peroxidase Complex (ABC): 1 x 130 uL
  • Sample Diluent Buffer: 1 x 30 mL
  • Antibody Diluent Buffer: 1 x 12 mL
  • ABC Diluent Buffer: 1 x 12 mL
  • TMB Color Developing Agent: 1 x 10 mL
  • TMB Stop Solution: 1 x 10 mL
  • 10X Wash Buffer: 1 x 30 mL
• Reconstitution and Storage: Store at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
• Sample Type: Cell culture supernatants, serum, plasma (heparin, EDTA) and urine
• Sensitivity: <150 pg/ml
• Specificity: Natural and recombinant mouse SAA
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay
• Immunogen: Expression system for standard: E.coli; Immunogen sequence: G20-Y122
• Description: For quantitative detection of mouse SAA in cell culture supernatants, serum, plasma(heparin, EDTA) and urine.

Target Info

• Gene Symbol: SAA1
• Gene Full Name: serum amyloid A1
• Alias Symbols: Amyloid fibril protein AA, Amyloid protein A, MGC111216, OTTMUSP00000045357, PIG4, SAA, SAA1, Serum amyloid A 1, Serum amyloid A 1 protein, serum amyloid A protein, Serum amyloid A protein precursor, Serum amyloid A1 isoform 1, Serum amyloid A1 isoform 2, TP53I4, Tumor protein p53 inducible protein 4
• NCBI Gene Id: 111345
• Protein Name: Serum amyloid A-1 protein
• Description of Target: Serum amyloid A protein (SAA), also known as SAA1, is a protein that in humans is encoded by the SAA1 gene. This gene encodes a member of the serum amyloid A family of apolipoproteins. It is mapped to 11p15.1. SAA is a major acute phase protein that is highly expressed in response to inflammation and tissue injury. This protein also plays an important role in HDL metabolism and cholesterol homeostasis. High levels of this protein are associated with chronic inflammatory diseases including atherosclerosis, rheumatoid arthritis, Alzheimer's disease and Crohn's disease. This protein may also be a potential biomarker for certain tumors.
• Uniprot ID: P05366
• Protein Accession #: NP_033143.1
• Nucleotide Accession #: NM_009117.3