SAA ELISA Kit (Human) (OKIA00083)

Catalog No: OKIA00083 (Formerly GWB-SAA374)

Size:1x96WELLPLATE

Sample Standard Curve is for demonstration only. Do not use for results calculations. A standard curve should be generated each time the test is performed.

Product Info

• Predicted Species Reactivity: Human
• Product Format: In Solution
• Application: ELISA
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: 90 Minutes
• ELISA Kit Principle: Aviva Systems Biology SAA ELISA Kit (Human) (OKIA00083) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody for Serum amyloid A-1 protein has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and washed to remove unbound proteins. An Anti-Serum amyloid A-1 protein - HRP conjugated detector antibody is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Serum amyloid A-1 protein captured in the well.
• ELISA Kit Range: 1.56 ng/ml - 100 ng/ml
• ELISA Kit Component:
  • Anti-Human SAA Microplate: 96 Wells (12 x 8 Well Strips)
  • 20X Diluent Concentrate: 50 mL
  • 20X Wash Solution: 50 mL
  • 100X Enzyme-Antibody Conjugate: 150 uL
  • Chromogen-Substrate Solution: 12 mL
  • Stop Solution: 12 mL
  • Human SAA Calibrator: 1
• Additional Information: Intended Use: The Human Serum Amyloid A (SAA) test kits are a highly sensitive two-site enzyme linked immunoassay (ELISA) for measuring SAA in serum or plasma samples of humans.
  Reagents:
  1. DILUENT CONCENTRATE (Running Buffer)
  One bottle containing 50 ml of a 5X concentrated diluent running buffer.
  2. WASH SOLUTION CONCENTRATE
  One bottle containing 50 ml of a 20X concentrated wash solution.
  3. ENZYME-ANTIBODY CONJUGATE 100X
  One vial containing 150 ul of affinity purified anti-Human SAA antibody conjugated with horseradish peroxidase in a stabilizing buffer.
  4. CHROMOGEN-SUBSTRATE SOLUTION
  One vial containing 12 mL of 3,3’,5,5’-tetramethybenzidine (TMB) and hydrogen peroxide in citric acid buffer at pH 3.3.
  5. STOP SOLUTION
  One vial containing 12 ml 0.3 M sulfuric acid.
  WARNING: Avoid contact with skin.
  6. ANTI-HUMAN SAA ELISA MICRO PLATE
  Twelve removable eight (8) well micro well strips in well holder frame. Each well is coated with affinity purified anti-Human SAA.
  7. HUMAN SAA CALIBRATOR
  One vial containing a lyophilized Human SAA calibrator.
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, plasma and other biological fluids
• Sensitivity: 0.492 ng/ml
• Predicted Homology Based on Immunogen Sequence: Human
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: SAA1
• Gene Full Name: serum amyloid A1
• Alias Symbols: SAA, PIG4, SAA2, TP53I4
• NCBI Gene Id: 6288
• Protein Name: Serum amyloid A-1 protein
• Description of Target: Major acute phase protein.
• Uniprot ID: P0DJI8