SAA ELISA Kit (Feline) (OKIA00197)

Catalog No: OKIA00197

Size:1 x 96 well plate

Representative Feline SAA Standard Curve plotted with four parameter logistic regression (4PL). Concentration is plotted as ng/mL.

Product Info

• Predicted Species Reactivity: Feline
• Product Format: 12 of 8-well strips
• Application: ELISA
• ELISA Kit Detection Method: Colorimetric, OD 450 nm
• ELISA Kit Duration: 90 Minutes
• ELISA Kit Principle: The Aviva Systems Biology SAA ELISA Kit (Feline) is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Feline Serum amyloid A protein in Plasma or Serum. This assay employs an antibody specific for Feline SAA coated on a 96-well plate. Standards and samples are pipetted into the wells and Serum amyloid A protein present in a sample is bound to the wells by the immobilized antibody. The wells are washed and anti-SAA antibodies conjugated with horseradish peroxidase (HRP) are added. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of SAA bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm. The quantity of SAA in the test sample can be interpolated from the standard curve constructed from the standards and corrected for sample dilution.
• ELISA Kit Range: 1.25 - 40 ng/mL
• ELISA Kit Reproducibility: < 10%
• ELISA Kit Component:
  • Anti-Feline SAA Microplate: 96 Wells (12 x 8 Well Strips)
  • Feline SAA Calibrator: 1
  • 100X SAA-HRP Conjugate: 150 uL
  • 5X Assay Diluent: 50 mL
  • 20X Wash Solution: 50 mL
  • Chromogen-Substrate Solution: 12 mL
  • Stop Solution: 12 mL
• Additional Information: Intended Use: The SAA test kits are a highly sensitive two-site enzyme linked immunoassay (ELISA) for measuring SAA/Serum amyloid A protein in Feline biological samples.
  Sample Volume: 5 uL
• Reconstitution and Storage: Store at 4 degrees
• Sensitivity: 0.8 ng/mL

Target Info

• Alias Symbols: SAA;Serum amyloid A protein;
• Protein Name: Serum amyloid A protein
• Description of Target: Major acute phase reactant. Apolipoprotein of the HDL complex.
• Uniprot ID: Q1T772