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Catalog No: OKIA00204
Size:1 x 96 well plate
Price: $500.00
SKU
OKIA00204
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityDog
Product Format12 of 8-well strips
ApplicationELISA
ELISA Kit Detection MethodColorimetric, OD 450 nm
ELISA Kit Duration100 Minutes
ELISA Kit PrincipleThe Aviva Systems Biology SAA ELISA Kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Canine Serum amyloid A protein in Plasma or Serum. This assay employs an antibody specific for Canine SAA coated on a 96-well plate. Standards and samples are pipetted into the wells and Serum amyloid A protein present in a sample is bound to the wells by the immobilized antibody. The wells are washed and anti-SAA antibodies conjugated with horseradish peroxidase (HRP) are added. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of SAA bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm. The quantity of SAA in the test sample can be interpolated from the standard curve constructed from the standards and corrected for sample dilution.
ELISA Kit Range6.25 - 200 ng/mL
ELISA Kit Reproducibility< 10%
ELISA Kit Component
ComponentAmount
Anti-Canine SAA Microplate96 Wells (12 x 8 Well Strips)
Canine SAA Calibrator1
100X SAA-HRP Conjugate150 uL
5X Assay Diluent50 mL
20X Wash Solution50 mL
Chromogen-Substrate Solution12 mL
Stop Solution12 mL
Additional InformationIntended Use: The SAA test kits are a highly sensitive two-site enzyme linked immunoassay (ELISA) for measuring SAA/Serum amyloid A protein in Canine biological samples.
::Sample Volume: 5 uL
Reconstitution and StorageStore at 4 degrees
Sensitivity2.6 ng/mL
Alias SymbolsSAA;Serum amyloid A protein;
Protein NameSerum amyloid A protein
Description of TargetMajor acute phase reactant. Apolipoprotein of the HDL complex.
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