S1PR3 ELISA Kit (Mouse) (OKEH03089)

Catalog No: OKEH03089

Size:96T

Mouse S1PR3 ELISA kit standard curve

Product Info

• Predicted Species Reactivity: Mouse, Mus musculus
• Application: Enzyme-linked Immunosorbent assay-Competitive
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva Systems Biology S1PR3 ELISA Kit (Mouse) (OKEH03089) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-mouse S1pr3 antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated S1pr3 and incubated. The S1pr3 found in the sample or standards competes with the biotinylated S1pr3 for limited binding sites on the immobilized anti-mouse S1pr3 antibody. Excess unbound biotinylated S1pr3 and sample or standard S1pr3 is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated S1pr3 captured in the well and inversely proportional to the amount of S1pr3 which was contained in the sample or standard.
• ELISA Kit Range: 0.312-20ng/mL
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 94%
• ELISA Kit Reproducibility: Mean Intra-assay CV%: <= 6.3% (n = 20)
  Mean Inter-assay CV%: <= 7.6% (n = 20)
• ELISA Kit Component:
  • Anti-Avp Microplate: 96 Wells (12 x 8 Well strips)
  • Avp Lyophilized Standard: 2
  • Sample Diluent: 1 x 20 mL
  • 100X Avp-Biotin Complex: 1 x 60 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Biotin Complex Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
• Sensitivity: 0.158 ng/mL
• Specificity: Natural and recombinant Mouse Sphingosine 1-phosphate receptor 3
• Assay Info: Assay Methodology: Quantitative Competitive ELISA

Target Info

• Gene Symbol: S1pr3
• Gene Full Name: sphingosine-1-phosphate receptor 3
• Alias Symbols: AI132464;Edg3;endothelial differentiation G-protein coupled receptor 3;endothelial differentiation sphingolipid G-protein-coupled receptor 3;LP;Lpb3;lysophospholipid receptor B3;S1;S1p receptor 3;S1P receptor Edg-3;S1p3;sphingosine 1-phosphate receptor 3;sphingosine 1-phosphate receptor Edg-3.
• NCBI Gene Id: 13610
• Protein Name: Q9Z0U9
• Description of Target: Sphingosine 1-phosphate receptor 3
• Uniprot ID: https://www.uniprot.org/uniprot/Q9Z0U9
• Protein Accession #: NP_034231.1
• Nucleotide Accession #: NM_010101.4
• Protein Size (# AA): 378
• Molecular Weight: 42 kDa