Catalog No: OKEH04291
Size:96T
Price: $525.00
SKU
OKEH04291
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
COA DatasheetPrintable COA datasheet for OKEH04291
Product Info
Predicted Species ReactivityHomo sapiens, Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology S1PR3 ELISA Kit (Human) (OKEH04291) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for S1PR3 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for S1PR3 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample S1PR3 captured in the well.
ELISA Kit Range0.156-10ng/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 99%
ELISA Kit ReproducibilityMean Intra-assay CV%: <= 4.3% (n = 20)
Mean Inter-assay CV%: <= 9.2% (n = 20)
ELISA Kit Component
ComponentAmount
S1PR3 Microplate96 Wells (12 x 8 Well Strips)
S1PR3 Standard2 x 2 ng
Sample Diluent1 x 20 mL
S1PR3 Biotinylated Detector Antibody1 x 120 uL
Detector Antibody Diluent1 x 12 mL
HRP Avidin Conjugate1 x 120 uL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue homogenates, Cell culture supernates and Other biological fluids.
Sensitivity0.078 ng/mL
SpecificityNatural and recombinant Human Sphingosine 1-phosphate receptor 3
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolS1PR3
Gene Full Namesphingosine-1-phosphate receptor 3
Alias SymbolsbA791O21.3;C9orf108;C9orf47;EDG3;EDG-3;endothelial differentiation G-protein coupled receptor 3;endothelial differentiation, sphingolipid G-protein-coupled receptor, 3;G protein-coupled receptor, endothelial differentiation gene-3;LPB3;S1P receptor 3;S1P receptor Edg-3;S1P receptor EDG3;S1P3;sphingosine 1-phosphate receptor 3;sphingosine 1-phosphate receptor Edg-3;uncharacterized protein C9orf47.
NCBI Gene Id1903
Protein NameQ99500
Description of TargetSphingosine 1-phosphate receptor 3
Uniprot IDhttps://www.uniprot.org/uniprot/Q99500
Protein Accession #NP_005217.2
Nucleotide Accession #NM_005226.3
Protein Size (# AA)378
Molecular Weight42 kDa
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