S1PR3 ELISA Kit (Human) (OKCD08689)

Catalog No: OKCD08689

Size:96WELLS

S1PR3 ELISA Kit (Human) (OKCD08689) Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Sphingosine 1 Phosphate Receptor 3 (S1PR3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Sphingosine 1 Phosphate Receptor 3 (S1PR3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Sphingosine 1 Phosphate Receptor 3 (S1PR3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Sphingosine 1 Phosphate Receptor 3 (S1PR3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 1.56-100ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Sphingosine 1 Phosphate Receptor 3 (S1PR3) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Sphingosine 1 Phosphate Receptor 3 (S1PR3) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • S1PR3 Microplate: 96 Wells (12 x 8 Well strips)
  • S1PR3 Lyophilized Standard: 2
  • 100X S1PR3 HRP-Detector Anitbody: 1 x 120 ÂuL
  • 100X Avidin-HRP Conjugate: 1 x 120 ÂuL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Tissue homogenates, cell lysates and other biological fluids
• Sensitivity: < 0.63ng/mL

Target Info

• Gene Symbol: S1PR3
• Gene Full Name: sphingosine-1-phosphate receptor 3
• Alias Symbols: bA791O21.3;C9orf108;C9orf47;EDG3;EDG-3;endothelial differentiation G-protein coupled receptor 3;endothelial differentiation, sphingolipid G-protein-coupled receptor, 3;G protein-coupled receptor, endothelial differentiation gene-3;LPB3;S1P receptor 3;S1P receptor Edg-3;S1P receptor EDG3;S1P3;sphingosine 1-phosphate receptor 3;sphingosine 1-phosphate receptor Edg-3;uncharacterized protein C9orf47.
• NCBI Gene Id: 1903
• Protein Name: Sphingosine 1-phosphate receptor 3
• Description of Target: Receptor for the lysosphingolipid sphingosine 1-phosphate (S1P). S1P is a bioactive lysophospholipid that elicits diverse physiological effect on most types of cells and tissues. When expressed in rat HTC4 hepatoma cells, is capable of mediating S1P-induced cell proliferation and suppression of apoptosis.
• Uniprot ID: Q99500
• Protein Accession #: NP_005217.2
• Nucleotide Accession #: NM_005226.3