Catalog No: OKEH00537 (Formerly GWB-DA1ZH8)
Size:96T
Price: $525.00
SKU
OKEH00537
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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S100B ELISA Kit (Mouse) : 96 Wells (OKEH00537)

Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
COA DatasheetPrintable COA datasheet for OKEH00537
Product Info
Predicted Species ReactivityMouse, Mus musculus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology S100B ELISA Kit (Mouse) (OKEH00537) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Mouse S100B has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. After washing,a biotinylated detector antibody specific for Mouse S100B is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Mouse S100B captured in well.
ELISA Kit Range15.6-1000pg/mL
ELISA Kit Recovery<= 5.9%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 3.9% (n = 20)
Mean Inter-assay CV%: < 6.7% (n = 20)
ELISA Kit Component
ComponentAmount
S100B Microplate96 Wells (12 x 8 Well strips)
S100B Lyophilized Standard2
100X Biotinylated S100B Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Sample Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Sensitivity7.8 pg/mL
SpecificityNatural and recombinant Mouse Protein S100-B
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolS100b
Gene Full NameS100 protein, beta polypeptide, neural
Alias SymbolsAI850290;Bpb;protein S100-B;S100 calcium-binding protein B;S-100 protein beta chain;S-100 protein subunit beta.
NCBI Gene Id20203
Protein NameP50114
Description of TargetProtein S100-B
Uniprot IDhttps://www.uniprot.org/uniprot/P50114
Protein Accession #NP_033141.1
Nucleotide Accession #NM_009115.3
Protein Size (# AA)92
Molecular Weight11 kDa
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