Aviva Systems Biology will be closed in observance of Good Friday 3/29/2024. If you are in need of any assistance please email info@avivasysbio.com

Catalog No: OKCA00128
Size:96 Wells
Price: $906.00
SKU
OKCA00128
Bulk
Order
Aviva's
Satisfaction
Guarantee
Contact Us:
Shipping Info:
  • $55: Antibody & Protein in US
  • $55 + $25/Kit in US
  • Contact us for international orders.

S100B ELISA Kit (Human) : 96 Wells (OKCA00128)

Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHuman
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration2.5 Hours
ELISA Kit Linearity
Sample Type 1
Sample TypeDilutionAverage %Range %
Serum (n=4)1:184%80-95%
Serum (n=4)1:289%85-94%
Serum (n=4)1:495%92-100%
Serum (n=4)1:890%85-98%
ELISA Kit PrincipleAviva Systems Biology S100B ELISA Kit (Human) (OKCA00128) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for human S100B has been pre-coated onto a 96-well plate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for human S100B is added, incubated and then followed by washing. Avidin-Biotin-Peroxidase Complex is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP producing a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample human S100B captured in well.
ELISA Kit Range78 pg/mL - 5,000 pg/mL
ELISA Kit Recovery
Sample 1
Sample TypeAverage RecoveryRange
Serum (n=5)95%85-99%
Sample 2
Sample TypeAverage RecoveryRange
EDTA Plasma (n=4)96%92-100%
ELISA Kit ReproducibilityIntra-assay: CV% < 8% (n = 20). Inter-assay: CV% < 10% (n = 20)
ELISA Kit Component
ComponentAmount
Anti-S100B Microplate96 Well (12 x 8 Well Strips)
S100B Standard (Lyophilized)2 x 5 ng
100X S100B Detection Antibody1 x 120 uL
100X HRP-Avidin1 x 120 uL
Detection Antibody Diluent1 x 15 mL
HRP-Avidin Diluent1 x 15 mL
Sample Diluent1 x 50 mL
25X Wash Buffer1 x 20 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue homogenates, Cerebrospinal fluid (CSF)
Sensitivity< 19.5 ng/mL
SpecificityThis assay employs the quantitative sandwich enzyme immunoassay technique. This assay has high sensitivity and excellent specificity for detection of human S-100B. No significant cross-reactivity or interference between human S-100B and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
Gene SymbolS100B
Gene Full NameS100 calcium binding protein B
Alias SymbolsNEF, S100, S100-B, S100beta
NCBI Gene Id6285
Protein NameProtein S100-B
Description of TargetThe protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21; however, this gene is located at 21q22.3. This protein may function in Neurite extension, proliferation of melanoma cells, stimulation of Ca2+ fluxes, inhibition of PKC-mediated phosphorylation, astrocytosis and axonal proliferation, and inhibition of microtubule assembly. Chromosomal rearrangements and altered expression of this gene have been implicated in several neurological, neoplastic, and other types of diseases, including Alzheimer's disease, Down's syndrome, epilepsy, amyotrophic lateral sclerosis, melanoma, and type I diabetes.
Uniprot IDP04271
Protein Accession #NP_006263
Nucleotide Accession #NM_006272
Protein Size (# AA)92
Molecular Weight11 kDa
Write Your Own Review
You're reviewing:S100B ELISA Kit (Human) : 96 Wells (OKCA00128)
Your Rating
We found other products you might like!