S100B Antibody (OAAB02489)

Catalog No: OAAB02489

Size:400ul

Western blot analysis of S100B Antibody in mouse liver tissue lysates (35ug/lane).S100B (arrow) was detected using the purified Pab.

Western blot analysis of lysates from human brain, mouse brain and rat brain tissue lysate (from left to right), using S100B Antibody(Cat. #OAAB02489). OAAB02489 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.

S100B Antibody (Cat. #OAAB02489)immunohistochemistry analysis in formalin fixed and paraffin embedded human malignant melanoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of S100B Antibody for immunohistochemistry. Clinical relevance has not been evaluated.

S100B Antibody (Cat. #OAAB02489) flow cytometric analysis of A375 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Product Info

• Predicted Species Reactivity: Human, Mouse
• Product Format: Liquid. PBS with 0.09% (W/V) sodium azide.
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Host: Rabbit
• Application: WB, IHC-P, FC
  Function: Weakly binds calcium but binds zinc very tightly- distinct binding sites with different affinities exist for both ions on each monomer. Physiological concentrations of potassium ion antagonize the binding of both divalent cations, especially affecting high-affinity calcium-binding sites. Binds to and initiates the activation of STK38 by releasing autoinhibitory intramolecular interactions within the kinase. Interaction with AGER after myocardial infarction may play a role in myocyte apoptosis by activating ERK1/2 and p53/TP53 signaling. Could assist ATAD3A cytoplasmic processing, preventing aggregation and favoring mitochondrial localization. May mediate calcium-dependent regulation on many physiological processes by interacting with other proteins, such as TPR-containing proteins, and modulating their activity.
  Cellular Location: Cytoplasm. Nucleus.
  Tissue Location: Although predominant among the water-soluble brain proteins, S100 is also found in a variety of other tissues.
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: This S100B antibody is generated from rabbits immunized with S100B recombinant protein.
• Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.
• Predicted Homology Based on Immunogen Sequence: Rabbit
• Concentration: Approximately 0.5mg/ml. Actual concentration varies with each lot.
• Application Info: WB: 1:1000
  IHC-P: 1:10~50
  FC: 1:10~50

Target Info

• Gene Symbol: S100B
• Alias Symbols: NEF, S100, S100-B, S100beta
• NCBI Gene Id: 6285
• Protein Name: Protein S100-B
• Description of Target: S100B is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21; however, this gene is located at 21q22.3. This protein may function in Neurite extension, proliferation of melanoma cells, stimulation of Ca2+ fluxes, inhibition of PKC-mediated phosphorylation, astrocytosis and axonal proliferation, and inhibition of microtubule assembly. Chromosomal rearrangements and altered expression of this gene have been implicated in several neurological, neoplastic, and other types of diseases, including Alzheimer's disease, Down's syndrome, epilepsy, amyotrophic lateral sclerosis, melanoma, and type I diabetes.
• Uniprot ID: P04271
• Protein Accession #: NP_006263.1
• Molecular Weight: 11 kDa