Catalog No: OKEH00564
Size:96 Wells
Price: $600.00
SKU
OKEH00564
Availability: Domestic: within 2-3 weeks delivery | International: 2-3 weeks
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S100A9 ELISA Kit (Human) : 96 Wells (OKEH00564)

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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
COA DatasheetPrintable COA datasheet for OKEH00564
Product Info
Predicted Species ReactivityHuman
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology S100A9 ELISA Kit (Human) (OKEH00564) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Human S100A9 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. After washing,a biotinylated detector antibody specific for Human S100A9 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Human S100A9 captured in well.
ELISA Kit Range78 - 5000pg/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 104%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 5.8% (n = 20)
Mean Inter-assay CV%: < 9.2% (n = 20)
ELISA Kit Component
ComponentAmount
S100A9 Microplate96 Wells (12 x 8 Well strips)
S100A9 Lyophilized Standard2
100X Biotinylated S100A9 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Sample Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Sensitivity19.5 pg/mL
SpecificityNatural and recombinant Human Protein S100-A9
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolS100A9
Gene Full NameS100 calcium binding protein A9
Alias SymbolsMIF, NIF, P14, CAGB, CFAG, CGLB, L1AG, LIAG, MRP14, 60B8AG, MAC387
NCBI Gene Id6280
Protein NameProtein S100-A9
Description of TargetCalcium-binding protein. Has antimicrobial activity towards bacteria and fungi. Important for resistance to invasion by pathogenic bacteria. Up-regulates transcription of genes that are under the control of NF-kappa-B. Plays a role in the development of endotoxic shock in response to bacterial lipopolysaccharide (LPS) (By similarity). Promotes tubulin polymerization when unphosphorylated. Promotes phagocyte migration and infiltration of granulocytes at sites of wounding. Plays a role as a pro-inflammatory mediator in acute and chronic inflammation and up-regulates the release of IL8 and cell-surface expression of ICAM1. Extracellular calprotectin binds to target cells and promotes apoptosis. Antimicrobial and proapoptotic activity is inhibited by zinc ions.
Swissprot IdP06702
Protein Accession #NP_002956.1
Nucleotide Accession #NM_002965.3
Protein Size (# AA)114
Molecular Weight13 kDa
Tissue ToolFind tissues and cell lines supported by DNA array analysis to express S100A9.
RNA SeqFind tissues and cell lines supported by RNA-seq analysis to express S100A9.
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