Catalog No: OKEH00564
Price: $600.00
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S100A9 ELISA Kit (Human) : 96 Wells (OKEH00564)

80% of 100
Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
COA DatasheetPrintable COA datasheet for OKEH00564
Product Info
Predicted Species ReactivityHomo sapiens, Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology S100A9 ELISA Kit (Human) (OKEH00564) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Human S100A9 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. After washing,a biotinylated detector antibody specific for Human S100A9 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Human S100A9 captured in well.
ELISA Kit Range78-5000pg/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 104%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 5.8% (n = 20)
Mean Inter-assay CV%: < 9.2% (n = 20)
ELISA Kit Component
S100A9 Microplate96 Wells (12 x 8 Well strips)
S100A9 Lyophilized Standard2
100X Biotinylated S100A9 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Sample Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Sensitivity19.5 pg/mL
SpecificityNatural and recombinant Human Protein S100-A9
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolS100A9
Gene Full NameS100 calcium binding protein A9
Alias Symbols60B8AG;CAGB;calgranulin B;Calgranulin-B;calprotectin L1H subunit;CFAG;CGLB;L1AG;leukocyte L1 complex heavy chain;LIAG;MAC387;MIF;migration inhibitory factor-related protein 14;MRP14;MRP-14;NIF;P14;protein S100-A9;S100 calcium-binding protein A9;S100-A9.
NCBI Gene Id6280
Protein NameP06702
Description of TargetProtein S100-A9
Uniprot IDhttps://www.uniprot.org/uniprot/P06702
Protein Accession #NP_002956.1
Nucleotide Accession #NM_002965.3
Protein Size (# AA)114
Molecular Weight13 kDa
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