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Datasheets/Manuals | Printable datasheet for S100A9 Antibody (OASA03072) |
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Predicted Species Reactivity | Human |
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Product Format | Liquid. Phosphate buffered saline |
Clonality | Monoclonal |
Clone | MAC387 |
Isotype | IgG1 |
Host | Mouse |
Application | Flow cytometry|Immunohistochemistry-Frozen|Immunohistochemistry-Paraffin |
Additional Information | Fusion Partners: Spleen cells from immunised BALB/c mice were fused with cells of the mouse NS1 myeloma cell line. Histology Positive Control Tissue: Human Spleen |
:: | Preparation: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant Preservative Stabilisers: 0.09% - Sodium Azide |
:: | Approx Protein Conc: IgG concentration 1.0 mg/ml Buffer Solutions: Phosphate buffered saline |
Reconstitution and Storage | -20°C |
Immunogen | Human monocytes. |
Purification | Protein A Purified |
Predicted Homology Based on Immunogen Sequence | Human |
Concentration | 1 mg/ml |
Specificity | OASA03072 ecognizes the L1 or Calprotectin molecule, an intracytoplasmic antigen comprised of a 12kD alpha chain and a 14kD beta chain. The antigen recognized by OASA03072 is expressed by granulocytes, monocytes and by tissue macrophages. Variable results have been reported for staining brain macrophages and microglia. |
Application Info | Flow Cytometry(1): 1/50 - 1/100 Immunohistology - Frozen: 1/100 - 1/200 Immunohistology - Paraffin(2): 1/100 - 1/200 (1)Membrane permeabilisation is required for this application. (2)This product requires protein digestion pre-treatment of paraffin sections e.g. trypsin or pronase. |
Reference | 1. Brandtzaeg, P. et al. (1988) MAC387 antibody and detection of formalin resistant myelomonocytic L1 antigen. J. Clin. Path. 41: 963-970. 2. Brandtzaeg, P. et al. (1992) The leucocyte protein L1 (calprotectin): usefulness as an immunohistochemical marker antigen and putative biological function. Histopathology. 21: 191-196. 3. Flavell, D.J. et al . (1987) Identification of tissue histiocytes on paraffin sections by a new monoclonal antibody. J. Histochem. Cytochem. 35: 1217-1226. 4. Gutierrez, M. et al. (1999) The detection of CD2+, CD4+, CD8+, and WC1+ T lymphocytes, B cells and macrophages in fixed and paraffin embedded bovine tissue using a range of antigen recovery and signal amplification techniques Vet. Immunol. Immunopathol. 71: 321-334. 5. Ramsay, A. D. et al. (1991) Phenotypic analysis of malignant lymphoma in simian immunodeficiency virus infection using anti-human antibodies. J. Pathol. 164: 321 - 328. 6. Christgau, M. et al. (1998) Characterization of Immunocompetent cells in the diseased canine periodontium. J. Histochem. Cytochem. 46: 1443 - 1454. 7. Perez, J. et al. (1999) Immunohistochemical study of the inflammatory infiltrate associated with equine squamous cell carcinoma J. Comp. Path. 121: 385 - 397. 8. Obert, L. et al. (2002) Early pathogenesis of transmucosal Feline Immunodeficiency Virus infection. J. Virol. 76: 6311 - 6322. 9. Malik, N. et al. (1998) Apoptosis and Cell proliferation after porcine coronary angioplasty. Circulation. 98: 1657 - 1665. 10. Bagavant, H. et al. (2002) Induction and immunohistology of autoimmune ovarian disease in cynomolgus macaques (Macaca fascicularis)). Am. J. Pathol. 160: 141 - 149. |
Gene Symbol | S100A9 |
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Alias Symbols | MIF, NIF, P14, CAGB, CFAG, CGLB, L1AG, LIAG, MRP14, 60B8AG, MAC387 |
NCBI Gene Id | 6280 |
Protein Name | Protein S100-A9 |
Uniprot ID | P06702 |
Protein Accession # | NP_002956.1 |
Protein Size (# AA) | 114 |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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See our General FAQ page.
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What is the species homology for "S100A9 Antibody (OASA03072)"?
The tested species reactivity for this item is "". This antibody is predicted to have homology to "Human".
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How long will it take to receive "S100A9 Antibody (OASA03072)"?
This item is available "Domestic: within 1-2 weeks delivery | International: 1-2 weeks".
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What buffer format is "S100A9 Antibody (OASA03072)" provided in?
This item is provided in "Liquid. Phosphate buffered saline".
Additional format options may be available. For more information please contact info@avivasysbio.com. -
What are other names for "S100A9 Antibody (OASA03072)"?
This target may also be called "MIF, NIF, P14, CAGB, CFAG, CGLB, L1AG, LIAG, MRP14, 60B8AG, MAC387" in publications.
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What is the shipping cost for "S100A9 Antibody (OASA03072)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
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What is the guarantee for "S100A9 Antibody (OASA03072)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
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Can I get bulk pricing for "S100A9 Antibody (OASA03072)"?
You can get bulk pricing for this item by going here.
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What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "".
Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics. -
What protocols are available for "S100A9 Antibody (OASA03072)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
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What are positive controls for "S100A9"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What are negative controls for "S100A9"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What other proteins interact with "S100A9"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
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What biological processes are associated with "S100A9"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
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What cellular components are associated with "S100A9"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
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What protein functions are associated with "S100A9"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.