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Catalog No: OKCD01096 (Formerly GWB-KBBPG4)
Size:96WELLS
Price: $750.00
SKU
OKCD01096
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Datasheets/ManualsPrintable datasheet for S100A7A ELISA Kit (Human) (OKCD01096)
Product Info
Predicted Species ReactivityHomo sapiens|Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit Linearity
Sample1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
serum(n=5)98-105%83-94%92-99%98-105%
EDTA plasma(n=5)82-91%84-101%88-102%88-102%
heparin plasma(n=5)81-104%99-105%80-102%97-105%
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to S100 Calcium Binding Protein A15 (S100A15). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to S100 Calcium Binding Protein A15 (S100A15). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain S100 Calcium Binding Protein A15 (S100A15), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of S100 Calcium Binding Protein A15 (S100A15) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.312-20ng/mL
ELISA Kit Recovery
MatrixRecovery Range (%)Average (%)
serum(n=5)84-10294
EDTA plasma(n=5)84-9490
heparin plasma(n=5)88-9894
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level S100 Calcium Binding Protein A15 (S100A15) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level S100 Calcium Binding Protein A15 (S100A15) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-S100A7A Microplate96 Wells (12 x 8 Well Strips)
S100A7A Lyophilized Standard2 x 20 ng
100X Biotinylated S100A7A Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Sensitivity< 0.113 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of S100 Calcium Binding Protein A15 (S100A15). No significant cross-reactivity or interference between S100 Calcium Binding Protein A15 (S100A15) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
Gene SymbolS100A7A
Gene Full NameS100 calcium binding protein A7A
Alias Symbolskoebnerisin;NICE2;NICE-2;protein S100-A7A;S100 calcium-binding protein A15;S100 calcium-binding protein A7A;S100 calcium-binding protein A7-like 1;S100A15;S100A7f;S100A7L1.
NCBI Gene Id338324
Protein NameProtein S100-A7A
Description of TargetMay be involved in epidermal differentiation and inflammation and might therefore be important for the pathogenesis of psoriasis and other diseases.
Uniprot IDQ86SG5
Protein Accession #NP_789793
Nucleotide Accession #NM_176823.3
Protein Size (# AA)101
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