S100A15A ELISA Kit (Mouse) (OKWB00258)

Catalog No: OKWB00258

Size:96T

Mouse S100A15A ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Mouse|Mus musculus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD 450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Linearity:

  Sample Type	1:2 Dilution	1:4 Dilution	1:8 Dilution	1:16 Dilution
  Serum (n=5)	89-105%	90-105%	87-105%	87-103%
  EDTA Plasma (n=5)	83-100%	86-94%	86-101%	90-99%
  Heparin Plasma (n=5)	81-100%	85-98%	88-98%	81-90%

• ELISA Kit Principle: Aviva Systems Biology S100a15a ELISA Kit (Mouse) (OKWB00258) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for S100a15a has been pre-coated onto a 96-well plate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for S100a15a is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample S100a15a captured in the well.
• ELISA Kit Range: 0.234-15ng/ml
• ELISA Kit Recovery:

  Sample Type	Range	Mean
  Serum (n=5)	85-99	90
  EDTA Plasma (n=5)	91-104	98
  Heparin Plasma (n=5)	88-105	95

• ELISA Kit Reproducibility: Mean Intra-Assay Precision <10%
  Mean Inter-Assay Precision <12%
• ELISA Kit Component:
  • S100a15a Microplate: 96 Wells (12 x 8 Well strips)
  • S100a15a Lyophilized Standard: 2 x 15 ng
  • 100X S100a15a Biotinylated Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Cell Cultures, Tissue Homogenates, Other Biological Fluids
• Sensitivity: 0.141 ng/mL
• Assay Info: Assay Type: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: S100a7a
• Gene Full Name: S100 calcium binding protein A7A
• Alias Symbols: AY465109;Gm1020;protein S100-A15A;Protein S100-A7A;S100 calcium binding protein A15;S100 calcium binding protein A7-like 1;S100 calcium-binding protein A15A;S100 calcium-binding protein A7A;S100A;S100a1;S100a15;S100a15a;S100a17l1;S100A7f;S100A7L1.
• NCBI Gene Id: 381493
• Protein Name: Protein S100-A15A
• Uniprot ID: Q6S5I3
• Protein Accession #: NP_955454
• Nucleotide Accession #: NM_199422