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Catalog No: OKCD00856 (Formerly GWB-KBBGI7)
Size:96WELLS
Price: $600.00
SKU
OKCD00856
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Datasheets/ManualsPrintable datasheet for S100A10 ELISA Kit (Rat) (OKCD00856)
Product Info
Predicted Species ReactivityRat|Rattus norvegicus
ApplicationELISA-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to S100 Calcium Binding Protein A10 (S100A10). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to S100 Calcium Binding Protein A10 (S100A10). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain S100 Calcium Binding Protein A10 (S100A10), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of S100 Calcium Binding Protein A10 (S100A10) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.78-50ng/mL
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level S100 Calcium Binding Protein A10 (S100A10) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level S100 Calcium Binding Protein A10 (S100A10) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-S100a10 Microplate96 Wells (12 x 8 Well Strips)
S100a10 Lyophilized Standard2 x 20 ng
100X Biotinylated S100a10 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeTissue homogenates, cell lysates and other biological fluids.
Sensitivity< 0.115 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of S100 Calcium Binding Protein A10 (S100A10). No significant cross-reactivity or interference between S100 Calcium Binding Protein A10 (S100A10) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
Gene SymbolS100a10
Gene Full NameS100 calcium binding protein A10
Alias Symbolsannexin II ligand;calpactin I light chain;calpactin-1 light chain;cellular ligand of annexin II;nerve growth factor-induced protein 42C;p10 protein;p11;protein S100-A10;S100 calcium-binding protein A10;S-100 related protein, clone 42C.
NCBI Gene Id81778
Protein NameProtein S100-A10
Description of TargetBecause S100A10 induces the dimerization of ANXA2/p36, it may function as a regulator of protein phosphorylation in that the ANXA2 monomer is the preferred target (in vitro) of tyrosine-specific kinase.
Uniprot IDP05943
Protein Accession #NP_112376
Nucleotide Accession #NM_031114.1
Protein Size (# AA)95
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