Catalog No: OKCD02824
Size:96WELLS
Price: $650.00
SKU
OKCD02824
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Datasheets/ManualsPrintable datasheet for S100A1 ELISA Kit (Rat) (OKCD02824)
Product Info
Predicted Species ReactivityRat|Rattus norvegicus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit Linearity
Sample1:21:41:81:16
Serum (n=5)80-97%98-105%79-92%96-103%
EDTA Plasma (n=5)88-96%92-104%82-99%82-96%
Heparin Plasma (n=5)86-99%94-102%91-105%80-90%
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to S100 Calcium Binding Protein (S100). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to S100 Calcium Binding Protein (S100). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain S100 Calcium Binding Protein (S100), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of S100 Calcium Binding Protein (S100) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.156-10ng/mL
ELISA Kit Recovery
MatrixRecovery range (%)Average(%)
Serum (n=5)89-9692
EDTA Plasma (n=5)88-9691
Heparin Plasma(n=5)92-10196
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level S100 Calcium Binding Protein (S100) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level S100 Calcium Binding Protein (S100) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-S100A1 Microplate96 Wells (12 x 8 Well strips)
S100A1 Lyophilized Standard2
100X Biotinylated S100A1 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
Stop Solution1 x 6 mL
TMB Substrate1 x 9 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma, tissue homogenates, cell lysates, cell culture supernatant and other biological fluids
Sensitivity0.061 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of S100 Calcium Binding Protein (S100). No significant cross-reactivity or interference between S100 Calcium Binding Protein (S100) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Protocol Information
Gene SymbolS100a1
Gene Full NameS100 calcium binding protein A1
Alias Symbolsprotein S100-A1;S100 calcium-binding protein A1;S-100 protein alpha chain;S-100 protein subunit alpha.
NCBI Gene Id295214
Protein NameProtein S100-A1
Description of TargetProbably acts as a Ca(2+) signal transducer (By similarity). In response to an increase in intracellular Ca(2+) levels, binds calcium which triggers a conformational change (PubMed:16169012, PubMed:18650434). This conformational change allows interaction of S1001A with specific target proteins, such as TPR-containing proteins, and the modulation of their activity (By similarity).
Uniprot IDP35467
Protein Accession #NP_001007637.1
Nucleotide Accession #NM_001007636.3
Protein Size (# AA)94
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