Catalog No: OKEH01702
Size:96T
Price: $600.00
SKU
OKEH01702
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHomo sapiens, Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology RUVBL1 ELISA Kit (Human) (OKEH01702) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for RUVBL1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for RUVBL1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample RUVBL1 captured in well.
ELISA Kit Range0.312-20ng/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 101%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 5.9% (n = 20)
Mean Inter-assay CV%: < 11.2% (n = 20)
ELISA Kit Component
ComponentAmount
RUVBL1 Microplate96 Wells (12 x 8 Well strips)
RUVBL1 Lyophilized Standard2
Sample Diluent1 x 20 mL
100X Biotinylated RUVBL1 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Sensitivity0.12 ng/mL
SpecificityNatural and recombinant Human RuvB-like 1
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolRUVBL1
Gene Full NameRuvB like AAA ATPase 1
Alias Symbols49 kDa TATA box-binding protein-interacting protein;49 kDa TBP-interacting protein;54 kDa erythrocyte cytosolic protein;ECP54;ECP-54;epididymis secretory sperm binding protein;INO80 complex subunit H;INO80H;NMP 238;NMP238;nuclear matrix protein 238;PONTIN;pontin 52;Pontin52;RuvB (E coli homolog)-like 1;ruvB-like 1;RuvB-like AAA ATPase;RVB1;TAP54-alpha;TATA binding protein interacting protein 49 kDa;TIH1;TIP49;TIP49A;TIP60-associated protein 54-alpha.
NCBI Gene Id8607
Protein NameQ9Y265
Description of TargetRuvB-like 1
Uniprot IDhttps://www.uniprot.org/uniprot/Q9Y265
Protein Accession #NP_001306013.1
Nucleotide Accession #NM_001319084.1
Protein Size (# AA)456
Molecular Weight50 kDa
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