RUNX3 Antibody - middle region (OAAB10994)

Catalog No: OAAB10994

Size:400ul

RUNX3 Antibody (Center) (Cat. #OAAB10994) western blot analysis in HL-60 cell line lysates (35ug/lane).This demonstrates the RUNX3 antibody detected the RUNX3 protein (arrow).

RUNX3 Antibody (Center) (OAAB10994)immunohistochemistry analysis in formalin fixed and paraffin embedded human stomach tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of RUNX3 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.

Fluorescent confocal image of Hela cell stained with RUNX3 Antibody (Center)(Cat#AP14667c).Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with RUNX3 primary antibody (1:25, 1 h at 37℃). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37℃).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37℃). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min). RUNX3 immunoreactivity is localized to Nucleus and Cytoplasm significantly.

Product Info

• Predicted Species Reactivity: Human
• Product Format: Liquid. PBS with 0.09% (W/V) sodium azide.
• Clonality: Polyclonal
• Isotype: Ig
• Host: Rabbit
• Application: WB, IHC-P, IF
  Function: CBF binds to the core site, 5'-PYGPYGGT-3', of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, lck, IL-3 and GM-CSF promoters.
  Cellular Location: Nucleus {ECO:0000255|PROSITE- ProRule:PRU00399, ECO:0000269|PubMed:20100835}. Cytoplasm. Note=The tyrosine phosphorylated form localizes to the cytoplasm.
  
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: This RUNX3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 168-197 amino acids from the Central region of human RUNX3.
• Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.
• Predicted Homology Based on Immunogen Sequence: Human
• Concentration: Approximately 0.5mg/ml. Actual concentration varies with each lot.
• Sample Type Confirmation:

  RUNX3 is strongly supported by BioGPS gene expression data to be expressed in HL60

• Intended Use: RUNX3 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
• Application Info: WB: 1:1000
  IHC-P: 1:10~50
  IF: 1:10~50

Target Info

• Gene Symbol: RUNX3
• Alias Symbols: AML2, CBFA3, PEBP2aC
• NCBI Gene Id: 864
• Protein Name: Runt-related transcription factor 3
• Description of Target: This gene encodes a member of the runt domain-containing family of transcription factors. A heterodimer of this protein and a beta subunit forms a complex that binds to the core DNA sequence 5'-PYGPYGGT-3' found in a number of enhancers and promoters, and can either activate or suppress transcription. It also interacts with other transcription factors. It functions as a tumor suppressor, and the gene is frequently deleted or transcriptionally silenced in cancer. Multiple transcript variants encoding different isoforms have been found for this gene.
• Uniprot ID: Q13761
• Protein Accession #: NP_001026850.1
• Molecular Weight: 44356 Da