RUNX1T1 Antibody (OADC00048)

Catalog No: OADC00048

Size:100ul

ChIP results obtained with the Aviva antibody directed against ETO. ChIP assays were performed using SKNO-1 cells, the Aviva antibody against ETO and optimized primer pairs for qPCR. Sheared chromatin from 1.25 million cells and 4 ul of antibody were used per ChIP experiment. QPCR was performed using primers specific for the FUT7, NFE2, OGG1 and VEGF genes. Figure shows the occupancy, calculated as the ratio + control/background for which the H2B gene was used.

ChIP-seq results obtained with the Aviva antibody directed against ETO r. ChIP was performed as described above. The IP'd DNA of 6 ChIP's was pooled and analysed with an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 32 bp tags were aligned to the human reference genome (hg18) using the ELAND algorithm. Figure shows the results of the complete chromosome 3 and three genomic regions surrounding the OGG1, FUT7 and NFE2 genes, respectively. The position of the PCR amplicon is indicated with an arrow.

Determination of the antibody titer. To determine the titer of the antibody, an ELISA was performed using a serial dilution of the Aviva antibody directed against human ETO. The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution the titer of the antibody was estimated to be 1:1,300.

Product Info

• Predicted Species Reactivity: Human
• Product Format: Liquid. Whole antiserum from rabbit containing 0.05% azide.
• Clonality: Polyclonal
• Host: Rabbit
• Conjugation: Unconjugated
• Application: CHIP
• Reconstitution and Storage: Store at -20C. Avoid repeated freeze/thaw cycles.
• Purification: Whole antiserum
• Concentration: Undetermined, ascites
• Preservative: 0.05% Sodium Azide
• Specificity: Polyclonal antibody raised in rabbit against human ETO (runt-related transcription factor 1; translocated to, 1 (cyclin D-related)) using two KLH-conjugated synthetic peptides containing sequences from the N-terminal and the central region of the protein, respectively.
• Application Info: CHIP: 4 ul/CHIP
  ELISA: 1:100

Target Info

• Gene Symbol: RUNX1T1
• Gene Full Name: RUNX1 translocation partner 1
• Alias Symbols: CDR, ETO, MTG8, AML1T1, ZMYND2, CBFA2T1, AML1-MTG8
• NCBI Gene Id: 862;
• Protein Name: protein CBFA2T1
• Description of Target: This gene encodes a member of the myeloid translocation gene family which interact with DNA-bound transcription factors and recruit a range of corepressors to facilitate transcriptional repression. The t(8;21)(q22;q22) translocation is one of the most frequent karyotypic abnormalities in acute myeloid leukemia. The translocation produces a chimeric gene made up of the 5'-region of the runt-related transcription factor 1 gene fused to the 3'-region of this gene. The chimeric protein is thought to associate with the nuclear corepressor/histone deacetylase complex to block hematopoietic differentiation. Alternative splicing results in multiple transcript variants.
• Uniprot ID: Q06455
• Protein Accession #: NP_001185554.1
• Nucleotide Accession #: NM_001198625.1