RUNX1 ELISA Kit (Human) (OKEH07609)

Catalog No: OKEH07609

Size:96T

Human RUNX1 ELISA Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens, Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Principle: Aviva Systems Biology RUNX1 ELISA Kit (Human) (OKEH07609) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for RUNX1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for RUNX1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample RUNX1 captured in well.
• ELISA Kit Range: 31.2-2000pg/mL
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 111% (n = 20)
• ELISA Kit Reproducibility: Mean Intra-assay CV%: <=7.2% (n = 20)
  Mean Inter-assay CV%: <=7.6% (n = 20)
• ELISA Kit Component:
  • RUNX1 Microplate: 96 Wells (12 x 8 Well strips)
  • RUNX1 Lyophilized Standard: 2
  • 100X Biotinylated RUNX1 Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Additional Information: Function: CBF binds to the core site, 5'-PYGPYGGT-3', of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, LCK, IL-3 and GM-CSF promoters. The alpha subunit binds DNA and appears to have a role in the development of normal hematopoiesis. Isoform AML-1L interferes with the transactivation activity of RUNX1. Acts synergistically with ELF4 to transactivate the IL-3 promoter and with ELF2 to transactivate the mouse BLK promoter. Inhibits KAT6B-dependent transcriptional activation. Controls the anergy and suppressive function of regulatory T-cells (Treg) by associating with FOXP3. Activates the expression of IL2 and IFNG and down-regulates the expression of TNFRSF18, IL2RA and CTLA4, in conventional T-cells (PubMed:17377532).
  Subcellular Location: Nucleus
  Unigene: Hs.149261
  SMR: Q01196
  String: 9606.ENSP00000300305
  KEGG: hsa:861
  MIM: 151385
  Pfam: PF00853
• Reconstitution and Storage: Store as indicated in product manual.
• Sensitivity: 10pg/mL
• Specificity: Natural and recombinant Human Runt-related transcription factor 1
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: RUNX1
• Gene Full Name: RUNX family transcription factor 1
• Alias Symbols: acute myeloid leukemia 1 protein;AML1;AML1-ETO fusion;AML1-ETO fusion protein;AML1-EVI-1;AML1-EVI-1 fusion protein;AMLCR1;CBF2alpha;CBFA2;Core-binding factor subunit alpha-2;core-binding factor, runt domain, alpha subunit 2;EVI-1;mutant RUNX1;oncogene AML-1;PEA2-alpha B;PEBP2aB;PEBP2alpha;PEBP2-alpha B;polyomavirus enhancer-binding protein 2 alpha B subunit;runt related transcription factor 1;runt-related transcription factor 1;SL3/AKV core-binding factor alpha B subunit;SL3-3 enhancer factor 1 alpha B subunit.
• NCBI Gene Id: 861
• Protein Name: Q01196
• Description of Target: Runt-related transcription factor 1
• Uniprot ID: https://www.uniprot.org/uniprot/Q01196
• Protein Accession #: NP_001001890.1
• Nucleotide Accession #: NM_001001890.2