RUNX1 Antibody - middle region (P100746_P050)

Catalog No: P100746_P050

WB Suggested Anti-RUNX1 Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1:12500
Positive Control: PANC1 cell lysateThere is BioGPS gene expression data showing that RUNX1 is expressed in PANC1

Product Info

• Tested Species Reactivity: Human
• Predicted Species Reactivity: Human, Mouse, Rat, Cow, Dog, Horse, Pig, Rabbit
• Product Format: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Clonality: Polyclonal
• Host: Rabbit
• Application: WB
• Reconstitution and Storage: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human RUNX1
• Purification: Affinity Purified
• Predicted Homology Based on Immunogen Sequence: Cow: 100%; Dog: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Pig: 100%; Rabbit: 100%; Rat: 100%
• Peptide Sequence: Synthetic peptide located within the following region: HPAPTPNPRASLNHSTAFNPQPQSQMQDTRQIQPSPPWSYDQSYQYLGSI
• Concentration: 0.5 mg/ml
• Blocking Peptide: For anti-RUNX1 (P100746_P050) antibody is Catalog # AAP31063 (Previous Catalog # AAPP01801)
• Sample Type Confirmation:

  There is BioGPS gene expression data showing that RUNX1 is expressed in PANC1

Target Info

• Reference: Takeshita,M., (2008) Leukemia 22 (6), 1241-1249
• Gene Symbol: RUNX1
• Gene Full Name: Runt-related transcription factor 1
• Alias Symbols: AML1, CBFA2, EVI-1, AMLCR1, PEBP2aB, CBF2alpha, AML1-EVI-1, PEBP2alpha
• NCBI Gene Id: 861
• Protein Name: Runt-related transcription factor 1
• Description of Target: Core binding factor (CBF) is a heterodimeric transcription factor that binds to the core element of many enhancers and promoters. RUNX1 is the alpha subunit of CBF and is thought to be involved in the development of normal hematopoiesis. Chromosomal translocations involving this gene are well-documented and have been associated with severaltypes of leukemia. Core binding factor (CBF) is a heterodimeric transcription factor that binds to the core element of many enhancers and promoters. The protein encoded by this gene represents the alpha subunit of CBF and is thought to be involved in the development of normal hematopoiesis. Chromosomal translocations involving this gene are well-documented and have been associated with several types of leukemia. Three transcript variants encoding different isoforms have been found for this gene.
• Uniprot ID: Q01196
• Protein Accession #: NP_001001890
• Nucleotide Accession #: NM_001001890
• Protein Size (# AA): 453
• Molecular Weight: 49kDa
• Protein Interactions: YAP1; UBC; HDAC11; SOX2; SMAD3; CTBP1; RUNX1; FOXP3; NCOR2; MYC; NOTCH1; UXT; CTBP2; KAT6A; PML; CREBBP; CBFB; PRMT1; HLA-DMA; HDAC2; HDAC1; CBFA2T3; RUNX1T1; CBFA2T2; ELAVL1; DNMT1; SPEN; RBM14; KMT2A; COPRS; HDAC3; TAL1; CDK1; SMARCC1; SMARCB1; SMARCA4;