Catalog No: OKDD00513 (Formerly GWB-KBBIY7)
Size:96 Wells
Price: $800.00
SKU
OKDD00513
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Datasheets/ManualsPrintable datasheet for RS ELISA Kit (Human) (OKDD00513)
Product Info
Predicted Species ReactivityHomo sapiens|Human
ApplicationEnzyme-linked immunosorbent assay
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~4 Hours
ELISA Kit PrincipleAviva Systems Biology RS ELISA Kit (Human) (OKDD00513) is based on standard sandwich enzyme-linked immuno-sorbent assay methodology. An antibody for RS has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for RS is added, incubated and removed. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample RS captured in well.
ELISA Kit Range0.156-10ng/mL
ELISA Kit Reproducibility

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level RS were tested 20 times on one plate, respectively.

Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level RS were tested on 3 different plates, 8 replicates in each plate.

Intra-Assay: CV<10%

Inter-Assay: CV<12%

ELISA Kit Component
ComponentAmount
Anti-RS Microplate96 Wells (12 x 8 Well Strips)
RS Lyophlized Standard2 x 10 ng
Standard Diluent1 x 20 mL
100X RS Biotinylated Detector Antibody1 x 120 uL
100X Avidin-HRP Detection Conjugate1 x 120 uL
Detector Antibody Diluent1 x 12 mL
Detection Conjugate Diluent1 x 12 mL
10X Sample Diluent1 x 30 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue homogenates, Cell lysates
Sensitivity< 0.069 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of RS. No significant cross-reactivity or interference between RS and analogues was observed.
Assay InfoQuantitative Sandwich ELISA
Gene SymbolRS1
Gene Full Nameretinoschisin 1
Alias Symbolsretinoschisin;RS;X-linked juvenile retinoschisis protein;XLRS1.
NCBI Gene Id6247
Protein NameRetinoschisin
Description of TargetBinds negatively charged membrane lipids, such as phosphatidylserine and phosphoinositides (By similarity). May play a role in cell-cell adhesion processes in the retina, via homomeric interaction between octamers present on the surface of two neighboring cells (PubMed:27114531). Required for normal structure and function of the retina (PubMed:19093009).
Uniprot IDO15537
Protein Accession #NP_000321.1
Nucleotide Accession #NM_000330.3
Protein Size (# AA)224
Molecular Weight26 kDa
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