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RRM2 Antibody - C-terminal region (ARP87047_P050)

100 ul
$289.00
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Tested Species Reactivity:
Human
Predicted Species Reactivity:
Human
Product Format:
Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Clonality:
Polyclonal
Host:
Rabbit
Application:
WB
Reconstitution and Storage:
For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
Gene Symbol:
RRM2
Official Gene Full Name:
ribonucleotide reductase regulatory subunit M2
NCBI Gene Id:
6241
Protein Name:
Ribonucleoside-diphosphate reductase subunit M2
Swissprot Id:
P31350
Protein Accession #:
NP_001025.1
Nucleotide Accession #:
NM_001034.3
Alias Symbols:
R2, RR2, RR2M
Description of Target:
This gene encodes one of two non-identical subunits for ribonucleotide reductase. This reductase catalyzes the formation of deoxyribonucleotides from ribonucleotides. Synthesis of the encoded protein (M2) is regulated in a cell-cycle dependent fashion. Transcription from this gene can initiate from alternative promoters, which results in two isoforms that differ in the lengths of their N-termini. Related pseudogenes have been identified on chromosomes 1 and X.
Protein Size (# AA):
389
Molecular Weight:
42 kDa
Purification:
Affinity purified
Tissue Tool:
Find tissues and cell lines supported by DNA array analysis to express RRM2.
RNA Seq:
Find tissues and cell lines supported by RNA-seq analysis to express RRM2.
Immunogen:
The immunogen is a synthetic peptide directed towards the C terminal region of human RRM2
Peptide Sequence:
Synthetic peptide located within the following region: QYIEFVADRLMLELGFSKVFRVENPFDFMENISLEGKTNFFEKRVGEYQR
Concentration:
Batch dependent within range: 100 ul at 0.5 - 1 mg/ml
Blocking Peptide:
For anti-RRM2 (ARP87047_P050) antibody is Catalog # AAP87047
Datasheets/Manuals:
Printable datasheet for anti-RRM2 (ARP87047_P050) antibody

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1 Item(s)

92/04/2019 06:48
  • Overall Experience:
  • Quality:
Human NT in WB

Submitted by:
Jesika Farido
University of the Pacific

RRM2

Sample type/lane description: Human NT

Lane 1: Human NT (1 hour)
Lane 2: Human NT + Doxo (1 hour)
Lane 3: Human NT + RRM2 Inhibitor (1 hour)
Lane 4: Human NT + Doxo+ inhibitor (1 hour)
Lane 5: Human NT (24 hours)
Lane 6: Human NT + Doxo (24 hours)
Lane 7: Human NT + RRM2 inhibitor (24 hours)
Lane 8: Human NT + D+ inhibitor (24 hours)

Protocol: Briefly, cells will be homogenized in lysis buffer (Cell Signaling) supplemented with aprotinin, leupeptin, and okadaic acid. Lysates will be clarified by centrifugation, and equal protein will be used for Western blotting. We will standardize loading based on GAPDH or another housekeeping protein. Secondary antibodies will be conjugated with IRDye 800CW or 680RD (LI-COR Biosciences) and proteins will be visualized with a LI-COR Odyssey Imager. 

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