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Catalog No: OKCD02855
Size:96WELLS
Price: $700.00
SKU
OKCD02855
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Datasheets/ManualsPrintable datasheet for RRM1 ELISA kit (Mouse)
Product Info
Predicted Species ReactivityMouse
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColormetric|Regular detection
ELISA Kit Duration3h
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Ribonucleotide Reductase M1 (RRM1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Ribonucleotide Reductase M1 (RRM1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Ribonucleotide Reductase M1 (RRM1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Ribonucleotide Reductase M1 (RRM1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.312-20ng/mL
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Ribonucleotide Reductase M1 (RRM1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Ribonucleotide Reductase M1 (RRM1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-RRM1 Microplate96 Wells (12 x 8 Well strips)
RRM1 Lyophilized Standard2
100X Biotinylated RRM1 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
Stop Solution1 x 6 mL
TMB Substrate1 x 9 mL
Reconstitution and Storage2°C to 8°C, -20°C
Sample TypeTissue homogenates and other biological fluids
Sensitivity0.124 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Ribonucleotide Reductase M1 (RRM1). No significant cross-reactivity or interference between Ribonucleotide Reductase M1 (RRM1) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Protocol Information
Gene SymbolRRM1
Gene Full Nameribonucleotide reductase M1
Alias SymbolsRn, RnrM1
NCBI Gene Id20133
Protein NameRibonucleoside-diphosphate reductase large subunit
Description of TargetProvides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides.
Uniprot IDP07742
Protein Accession #NP_033129.2
Nucleotide Accession #NM_009103.3
Protein Size (# AA)792
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