RRAGA ELISA Kit (Mouse) (OKEH08550)

Catalog No: OKEH08550

Size:96T

Mouse RRAGA ELISA Standard Curve

Product Info

• Predicted Species Reactivity: Mouse, Mus musculus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Principle: Aviva Systems Biology RRAGA ELISA Kit (Mouse) (OKEH08550) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for RRAGA has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for RRAGA is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample RRAGA captured in well.
• ELISA Kit Range: 0.312-20ng/mL
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 99% (n = 20)
• ELISA Kit Reproducibility: Mean Intra-assay CV%: <=5.1% (n = 20)
  Mean Inter-assay CV%: <=11.2% (n = 20)
• ELISA Kit Component:
  • RRAGA Microplate: 96 Wells (12 x 8 Well strips)
  • RRAGA Lyophilized Standard: 2
  • 100X Biotinylated RRAGA Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Additional Information: Function: Guanine nucleotide-binding protein that plays a crucial role in the cellular response to amino acid availability through regulation of the mTORC1 signaling cascade. Forms heterodimeric Rag complexes with RRAGC or RRAGD and cycles between an inactive GDP-bound and an active GTP-bound form. In its active form participates in the relocalization of mTORC1 to the lysosomes and its subsequent activation by the GTPase RHEB. Involved in the RCC1/Ran-GTPase pathway. May play a direct role in a TNF-alpha signaling pathway leading to induction of cell death.
  Subcellular Location: Cytoplasm Nucleus Lysosome Predominantly cytoplasmic. May shuttle between the cytoplasm and nucleus, depending on the bound nucleotide state (By similarity).
  Unigene: Mm.31178
  SMR: Q80X95
  String: 10090.ENSMUSP00000088591
  KEGG: mmu:68441
  Pfam: PF04670
• Reconstitution and Storage: Store as indicated in product manual.
• Sensitivity: 0.099ng/mL
• Specificity: Natural and recombinant Mouse Ras-related GTP-binding protein A
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: Rraga
• Gene Full Name: Ras-related GTP binding A
• Alias Symbols: 1300010C19Rik;AI255374;FIP;FIP-1;rag A;RAGA;ras-related GTP-binding protein A.
• NCBI Gene Id: 68441
• Protein Name: Q80X95
• Description of Target: Ras-related GTP-binding protein A
• Uniprot ID: https://www.uniprot.org/uniprot/Q80X95
• Protein Accession #: NP_848463.1
• Nucleotide Accession #: NM_178376.3