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Catalog No: OKEH01117
Price: $525.00
Availability: Domestic: within 2-3 weeks delivery | International: 2-3 weeks
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RPTOR ELISA Kit (Human) : 96 Wells (OKEH01117)

Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHomo sapiens, Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology RPTOR ELISA Kit (Human) (OKEH01117) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for RPTOR has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for RPTOR is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample RPTOR captured in well.
ELISA Kit Range0.312-20ng/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 95%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 3.9% (n = 20)
Mean Inter-assay CV%: < 7.1% (n = 20)
ELISA Kit Component
RPTOR Microplate96 Wells (12 x 8 Well strips)
RPTOR Lyophilized Standard2
100X Biotinylated RPTOR Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Sample Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
Sensitivity0.1 ng/mL
SpecificityNatural and recombinant Human Regulatory-associated protein of mTOR
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolRPTOR
Gene Full Nameregulatory associated protein of MTOR complex 1
Alias SymbolsKOG1;Mip1;p150 target of rapamycin (TOR)-scaffold protein;p150 target of rapamycin (TOR)-scaffold protein containing WD-repeats;raptor;regulatory-associated protein of mTOR.
NCBI Gene Id57521
Protein NameQ8N122
Description of TargetRegulatory-associated protein of mTOR
Uniprot ID
Protein Accession #NP_001156506.1
Nucleotide Accession #NM_001163034.1
Protein Size (# AA)1335
Molecular Weight149 kDa
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