Size:400 ul
$329.00
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SKU
OAAB18665
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More Information
Predicted Species Reactivity Human, Mouse, Rat
Product Format Liquid. PBS with 0.09% (W/V) sodium azide.
Clonality Monoclonal
Clone 1411CT316.2.151.34
Isotype IgG1, k
Host Mouse
Application IHC-P, WB,
Additional Information Cellular Location: Cytoplasm. Lysosome. Cytoplasmic granule. Note=Targeting to lysosomes depends on amino acid availability. In arsenite-stressed cells, accumulates in stress granules when associated with SPAG5 and association with lysosomes is drastically decreased.
Tissue Location Highly expressed in skeletal muscle, and in a lesser extent in brain, lung, small intestine, kidney and placenta. Isoform 3 is widely expressed, with highest levels in nasal mucosa and pituitary and lowest in spleen.
Reconstitution and Storage Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
Replacement Item This antibody may replace item sc-81537 from Santa Cruz Biotechnology.
Immunogen This RPTOR antibody is generated from a mouse immunized with recombinant protein.
Purification This antibody is purified through a protein G column, followed by dialysis against PBS.
Datasheets/Manuals Printable datasheet for anti-RPTOR (OAAB18665) antibody
Application Info IHC-P: 1:25
WB: 1:500~1:1000
Application Data WB 1:500~1000
IHC-P 1:25
Intended Use For research use only and not for use in diagnostic or therapeutic procedures.
Target Reference Kim D.-H.,et al.Cell 110:163-175(2002).
Hara K.,et al.Cell 110:177-189(2002).
Zody M.C.,et al.Nature 440:1045-1049(2006).
Mural R.J.,et al.Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
Nagase T.,et al.DNA Res. 7:65-73(2000).
Gene Symbol RPTOR
Alias Symbols Regulatory-associated protein of mTOR, Raptor, p150 target of rapamycin (TOR)-scaffold protein, RPTOR, KIAA1303, RAPTOR
NCBI Gene Id 57521
Description of Target Involved in the control of the mammalian target of rapamycin complex 1 (mTORC1) activity which regulates cell growth and survival, and autophagy in response to nutrient and hormonal signals; functions as a scaffold for recruiting mTORC1 substrates. mTORC1 is activated in response to growth factors or amino acids. Growth factor-stimulated mTORC1 activation involves a AKT1- mediated phosphorylation of TSC1-TSC2, which leads to the activation of the RHEB GTPase that potently activates the protein kinase activity of mTORC1. Amino acid-signaling to mTORC1 requires its relocalization to the lysosomes mediated by the Ragulator complex and the Rag GTPases. Activated mTORC1 up-regulates protein synthesis by phosphorylating key regulators of mRNA translation and ribosome synthesis. mTORC1 phosphorylates EIF4EBP1 and releases it from inhibiting the elongation initiation factor 4E (eiF4E). mTORC1 phosphorylates and activates S6K1 at 'Thr-389', which then promotes protein synthesis by phosphorylating PDCD4 and targeting it for degradation. Involved in ciliogenesis.
Swissprot Id Q8N122
Molecular Weight 149 kDa
Tissue Tool Find tissues and cell lines supported by DNA array analysis to express RPTOR.
RNA Seq Find tissues and cell lines supported by RNA-seq analysis to express RPTOR.
  1. What is the species homology for "RPTOR Antibody (OAAB18665)"?

    The tested species reactivity for this item is "". This antibody is predicted to have homology to "Human, Mouse, Rat".

  2. How long will it take to receive "RPTOR Antibody (OAAB18665)"?

    This item is available "Domestic: within 1 week delivery | International: 1 week".

  3. What buffer format is "RPTOR Antibody (OAAB18665)" provided in?

    This item is provided in "Liquid. PBS with 0.09% (W/V) sodium azide. ".
    Additional format options may be available. For more information please contact info@avivasysbio.com.

  4. What are other names for "RPTOR Antibody (OAAB18665)"?

    This target may also be called "Regulatory-associated protein of mTOR, Raptor, p150 target of rapamycin (TOR)-scaffold protein, RPTOR, KIAA1303, RAPTOR" in publications.

  5. What is the shipping cost for "RPTOR Antibody (OAAB18665)"?

    The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.

  6. What is the guarantee for "RPTOR Antibody (OAAB18665)"?

    All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.

  7. Can I get bulk pricing for "RPTOR Antibody (OAAB18665)"?

    You can get bulk pricing for this item by going here.

  8. What is the molecular weight of the protein?

    The molecular weight reported by Uniprot for this item is "149 kDa".
    Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics.

  9. What protocols are available for "RPTOR Antibody (OAAB18665)"?

    We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data tab on the product page.

  10. What are positive controls for "RPTOR"?

    We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.

  11. What are negative controls for "RPTOR"?

    We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.

  12. What other proteins interact with "RPTOR"?

    This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.

  13. What biological processes are associated with "RPTOR"?

    This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.

  14. What cellular components are associated with "RPTOR"?

    This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.

  15. What protein functions are associated with "RPTOR"?

    This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.

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