RPTOR Antibody (OAAB18665)

Catalog No: OAAB18665

Size:400ul

Western blot analysis of lysates from Hela, MCF-7, mouse C2C12, mouse NIH/3T3, rat C6 cell line (from left to right), using RPTOR Antibody (Cat # OAAB18665). OAAB18665 was diluted at 1: 1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1: 10000 dilution was used as the secondary antibody. Lysates at 20ug per lane.

Immunohistochemical analysis of paraffin-embedded H. skeletal muscle section using RPTOR Antibody (Cat # OAAB18665). OAAB18665 was diluted at 1: 25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.

Product Info

• Predicted Species Reactivity: Human, Mouse, Rat
• Product Format: Liquid. PBS with 0.09% (W/V) sodium azide.
• Clonality: Monoclonal
• Clone: 1411CT316.2.151.34
• Isotype: IgG1, k
• Host: Mouse
• Application: IHC-P, WB,
• Additional Information: Cellular Location: Cytoplasm. Lysosome. Cytoplasmic granule. Note=Targeting to lysosomes depends on amino acid availability. In arsenite-stressed cells, accumulates in stress granules when associated with SPAG5 and association with lysosomes is drastically decreased.
  Tissue Location Highly expressed in skeletal muscle, and in a lesser extent in brain, lung, small intestine, kidney and placenta. Isoform 3 is widely expressed, with highest levels in nasal mucosa and pituitary and lowest in spleen.
  
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: This RPTOR antibody is generated from a mouse immunized with recombinant protein.
• Purification: This antibody is purified through a protein G column, followed by dialysis against PBS.
• Application Info: IHC-P: 1:25
  WB: 1:500~1:1000
• Application Data: WB 1:500~1000
  IHC-P 1:25
• Intended Use: For research use only and not for use in diagnostic or therapeutic procedures.
• Reference: Kim D.-H.,et al.Cell 110:163-175(2002).
  Hara K.,et al.Cell 110:177-189(2002).
  Zody M.C.,et al.Nature 440:1045-1049(2006).
  Mural R.J.,et al.Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
  Nagase T.,et al.DNA Res. 7:65-73(2000).
  

Target Info

• Gene Symbol: RPTOR
• Alias Symbols: KOG1, Mip1
• NCBI Gene Id: 57521
• Description of Target: Involved in the control of the mammalian target of rapamycin complex 1 (mTORC1) activity which regulates cell growth and survival, and autophagy in response to nutrient and hormonal signals; functions as a scaffold for recruiting mTORC1 substrates. mTORC1 is activated in response to growth factors or amino acids. Growth factor-stimulated mTORC1 activation involves a AKT1- mediated phosphorylation of TSC1-TSC2, which leads to the activation of the RHEB GTPase that potently activates the protein kinase activity of mTORC1. Amino acid-signaling to mTORC1 requires its relocalization to the lysosomes mediated by the Ragulator complex and the Rag GTPases. Activated mTORC1 up-regulates protein synthesis by phosphorylating key regulators of mRNA translation and ribosome synthesis. mTORC1 phosphorylates EIF4EBP1 and releases it from inhibiting the elongation initiation factor 4E (eiF4E). mTORC1 phosphorylates and activates S6K1 at 'Thr-389', which then promotes protein synthesis by phosphorylating PDCD4 and targeting it for degradation. Involved in ciliogenesis.
• Uniprot ID: Q8N122
• Molecular Weight: 149 kDa