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Catalog No: OKWB00213
Size:96 Wells
Price: $450.00
SKU
OKWB00213
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHuman
ELISA Kit Detection MethodColorimetric, OD 450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit Linearity
Sample Type1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
Serum (n=5)88-102%87-95%86-102%85-105%
EDTA Plasma (n=5)83-101%91-99%93-99%82-101%
Heparin Plasma (n=5)84-97%86-100%80-97%80-96%
ELISA Kit PrincipleAviva Systems Biology RPS27A ELISA Kit (Human) (OKWB00213) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for RPS27A has been pre-coated onto a 96-well plate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for RPS27A is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample RPS27A captured in the well.
ELISA Kit Range0.063 - 4 ng/mL
ELISA Kit Recovery
Sample TypeRangeMean
Serum (n=5)91-9693
EDTA Plasma (n=5)89-9693
Heparin Plasma (n=5)95-10398
ELISA Kit ReproducibilityMean Intra-Assay Precision <10%
Mean Inter-Assay Precision <12%
ELISA Kit Component
ComponentAmount
RPS27A Microplate96 Wells (12 x 8 Well strips)
RPS27A Lyophilized Standard2 x 4 ng
100X RPS27A Biotinylated Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 20 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Cell Cultures, Tissue Homogenates, Other Biological Fluids
Sensitivity37.5 pg/mL
Assay InfoAssay Type: Quantitative Sandwich ELISA
Gene SymbolRPS27A
Alias SymbolsUBC, S27A, CEP80, UBA80, HEL112, UBCEP1, UBCEP80
NCBI Gene Id6233
Protein NameUbiquitin-40S ribosomal protein S27a
Description of TargetUbiquitin: Exists either covalently attached to another protein, or free (unanchored). When covalently bound, it is conjugated to target proteins via an isopeptide bond either as a monomer (monoubiquitin), a polymer linked via different Lys residues of the ubiquitin (polyubiquitin chains) or a linear polymer linked via the initiator Met of the ubiquitin (linear polyubiquitin chains). Polyubiquitin chains, when attached to a target protein, have different functions depending on the Lys residue of the ubiquitin that is linked: Lys-6-linked may be involved in DNA repair; Lys-11-linked is involved in ERAD (endoplasmic reticulum-associated degradation) and in cell-cycle regulation; Lys-29-linked is involved in lysosomal degradation; Lys-33-linked is involved in kinase modification; Lys-48-linked is involved in protein degradation via the proteasome; Lys-63-linked is involved in endocytosis, DNA-damage responses as well as in signaling processes leading to activation of the transcription factor NF-kappa-B. Linear polymer chains formed via attachment by the initiator Met lead to cell signaling. Ubiquitin is usually conjugated to Lys residues of target proteins, however, in rare cases, conjugation to Cys or Ser residues has been observed. When polyubiquitin is free (unanchored-polyubiquitin), it also has distinct roles, such as in activation of protein kinases, and in signaling. 40S Ribosomal protein S27a: Component of the 40S subunit of the ribosome.
Uniprot IDP62979
Protein Accession #NP_001129064
Nucleotide Accession #NM_001135592
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