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Datasheets/Manuals | Printable datasheet for anti-RpS27A (OAED00134) antibody |
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Predicted Species Reactivity | Human, Mouse, Rat, Bovine, Dog, Chicken, Drosophila, Fish, Guinea Pig, Hamster, Monkey, Pig, Rabbit, Sheep, Xenopus, Yeast |
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Clonality | Polyclonal |
Host | Rabbit |
Application | FC |
Reconstitution and Storage | Long Term Storage : -20C |
Immunogen | Native bovine Ubiquitin. |
Specificity | Recognizes human, mouse, rat, bovine, dog, chicken, Drosophila, fish, guinea pig, hamster, monkey, pig, rabbit, sheep, Xenopus and yeast Ubiquitin. Detects a band of ~10kDa by Western blot. |
Formulation | Liquid. In PBS containing 0.09% sodium azide. |
Purity | Protein A affinity purified. |
Application Info | Flow Cytometry (1:20) Optimal conditions must be determined individually for each application. |
Gene Symbol | RpS27A |
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Gene Full Name | Ubiquitin-40S ribosomal protein S27a |
Alias Symbols | CG5271, Dmel\CG5271, Dub80, DUb80, l(2)04820, l(2)31Ei, mfs(2)31, mfs(2)48, mfs48, RpS27, UB3-D, Ubi-f80, Ubi-m, Ubiq |
NCBI Gene Id | 34420 |
Protein Name | Ubiquitin-40S ribosomal protein S27a |
Description of Target | Ubiquitin is a 76-amino acid polypeptide expressed in all eukaryotic cells, highly conserved from yeast to humans. The modification of proteins by chains of ubiquitin mediates targeting of cytosolic and nuclear proteins for degradation by proteasomes. Ubiquitination represents an essential cellular process affected by a multi-enzyme cascade involving classes of enzymes known as ubiquitin-activating enzymes (E1s), ubiquitin-conjugating enzymes (E2s or Ubcs) and ubiquitin-protein ligases (E3s). E1 activates ubiquitin in an ATP-dependent manner with the formation of a thiol-ester linkage between the carboxy terminus of ubiquitin and E1. Sequential, transient thiol-ester bonds then generate between the carboxyl terminus of ubiquitin and specific cysteines of E2 and E3 enzymes. These bonds culminate in the formation of an isopeptide linkage between the activated carboxy terminus of ubiquitin and the epsilon-amino group of a lysine on a target protein, or within another ubiquitin chain (usually K48 of ubiquitin) resulting in the generation of chains of ubiquitin (polyubiquitin or multiubiquitin) from 4-20 ubiquitin moieties. Although the formation of K48-linked polyubiquitin chains on proteins constitutes a potent targeting signal for degradation in 26S proteasomes, ubiquitination is also reversible, and a number of not fully characterized deubiquitinating enzymes exist free in the cytosol. Research exploring how proteasome targets ER-retained proteins for degradation suggests that ER chaperones (such as Cne1p/calnexin, Kar2p/BiP or other components of the protein processing or transport machineries) helped by cytosolic chaperones (such as members of the Hsp70 and DnaJ families) recognize, target and force misfolded, unassembled or aberrantly modified proteins through the translocon. The retrotranslocated polypeptide chains are then released into the cytosol as soluble proteins or remain adhered to the cytosolic face of the ER membrane where deglycosylation and polyubiquitination followed by binding to the proteasome as well as degradation to peptides occurs. Ubiquitination also participates in the internalization and degradation of plasma membrane proteins such as some of the TCR subunits while still ER-membrane associated. |
Uniprot ID | P15357 |
Protein Accession # | NP_476778.1 |
Nucleotide Accession # | NM_057430.4 |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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See our General FAQ page.
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What is the species homology for "RpS27A Antibody (OAED00134)"?
The tested species reactivity for this item is "". This antibody is predicted to have homology to "Human, Mouse, Rat, Bovine, Dog, Chicken, Drosophila, Fish, Guinea Pig, Hamster, Monkey, Pig, Rabbit, Sheep, Xenopus, Yeast".
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How long will it take to receive "RpS27A Antibody (OAED00134)"?
This item is available "Domestic: within 1-2 week delivery | International: 1-2 week".
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What buffer format is "RpS27A Antibody (OAED00134)" provided in?
This item is provided in "".
Additional format options may be available. For more information please contact info@avivasysbio.com. -
What are other names for "RpS27A Antibody (OAED00134)"?
This target may also be called "CG5271, Dmel\CG5271, Dub80, DUb80, l(2)04820, l(2)31Ei, mfs(2)31, mfs(2)48, mfs48, RpS27, UB3-D, Ubi-f80, Ubi-m, Ubiq" in publications.
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What is the shipping cost for "RpS27A Antibody (OAED00134)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
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What is the guarantee for "RpS27A Antibody (OAED00134)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
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Can I get bulk pricing for "RpS27A Antibody (OAED00134)"?
You can get bulk pricing for this item by going here.
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What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "".
Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics. -
What protocols are available for "RpS27A Antibody (OAED00134)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
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What are positive controls for "RPS27A"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What are negative controls for "RPS27A"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What other proteins interact with "RPS27A"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
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What biological processes are associated with "RPS27A"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
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What cellular components are associated with "RPS27A"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
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What protein functions are associated with "RPS27A"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.