Catalog No: OKEH06343
Size:96T
Price: $600.00
SKU
OKEH06343
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
Please contact customer service when ordering 10+ kits.
Contact Us:
- Toll Free: 888-880-0001
- Phone: 858-552-6979
- Email: info@avivasysbio.com
Shipping Info:
- $55: Antibody & Protein in US
- $55 + $25/Kit in US
- Contact us for international orders.
Datasheets/Manuals | Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit. |
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Predicted Species Reactivity | Homo sapiens, Human | ||||||||||||||||||||||
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Application | Enzyme-linked Immunosorbent assay-Sandwich | ||||||||||||||||||||||
ELISA Kit Detection Method | Colorimetric, OD450 nm | ||||||||||||||||||||||
ELISA Kit Duration | ~3 Hours | ||||||||||||||||||||||
ELISA Kit Principle | Aviva Systems Biology RPS14 ELISA Kit (Human) (OKEH06343) is based on a competitive enzyme immunoassay technique. The microtiter well-plate in this kit has been pre-coated with an anti-RPS14 antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated RPS14 and incubated. The RPS14 found in the sample or standards competes with the biotinylated RPS14 for limited binding sites on the immobilized anti-RPS14 antibody. Excess unbound biotinylated RPS14 and sample or standard RPS14 is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated RPS14 captured in the well and inversely proportional to the amount of RPS14 which was contained in the sample or standard. | ||||||||||||||||||||||
ELISA Kit Range | 0.312-20ng/mL | ||||||||||||||||||||||
ELISA Kit Recovery | Mean recovery when spiking into Serum and Plasma = 94% | ||||||||||||||||||||||
ELISA Kit Reproducibility | Three samples concentrations were measured in replicate within an assay plate and across replicate assays to assess Mean Intra- and Mean Inter-Assay Precision. Mean Intra-Assay Precision - <= 7.4% (n = 20) Mean Inter-Assay Precision - <= 8.7% (n = 20) | ||||||||||||||||||||||
ELISA Kit Component |
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Reconstitution and Storage | Store as indicated in product manual. | ||||||||||||||||||||||
Sample Type | Serum, plasma and other biological fluids | ||||||||||||||||||||||
Sensitivity | 0.175 ng/mL | ||||||||||||||||||||||
Specificity | Natural and recombinant Human 40S ribosomal protein S14 | ||||||||||||||||||||||
Assay Info | Assay Methodology: Quantitative Sandwich ELISA |
Gene Symbol | RPS14 |
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Gene Full Name | ribosomal protein S14 |
Alias Symbols | 40S ribosomal protein S14;emetine resistance;EMTB;S14;small ribosomal subunit protein uS11. |
NCBI Gene Id | 6208 |
Protein Name | P62263 |
Description of Target | 40S ribosomal protein S14 |
Uniprot ID | https://www.uniprot.org/uniprot/P62263 |
Protein Accession # | NP_839972 |
Nucleotide Accession # | NM_178241 |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
-
See our General FAQ page.
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