RPN1 ELISA Kit (Human) (OKCD00686)

Catalog No: OKCD00686 (Formerly GWB-KBBTA5)

Size:96WELLS

Human RPN1 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Ribophorin I (RPN1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Ribophorin I (RPN1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Ribophorin I (RPN1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Ribophorin I (RPN1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 1.56-100ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Ribophorin I (RPN1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Ribophorin I (RPN1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-RPN1 Microplate: 96 Wells (12 x 8 Well Strips)
  • RPN1 Lyophilized Standard: 2 x 100 ng
  • 100X Biotinylated RPN1 Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Tissue homogenates and other biological fluids.
• Sensitivity: < 0.63 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Ribophorin I (RPN1). No significant cross-reactivity or interference between Ribophorin I (RPN1) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: RPN1
• Gene Full Name: ribophorin I
• Alias Symbols: dolichyl-diphosphooligosaccharide-protein glycosyltransferase 67 kDa subunit;Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 67 kDa subunit;dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1;oligosaccharyltransferase 1 homolog;oligosaccharyltransferase complex subunit (non-catalytic);OST1;RBPH1;Ribophorin I;ribophorin-1;RPN-I.
• NCBI Gene Id: 6184
• Protein Name: Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1
• Description of Target: Essential subunit of the N-oligosaccharyl transferase (OST) complex which catalyzes the transfer of a high mannose oligosaccharide from a lipid-linked oligosaccharide donor to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains.
• Uniprot ID: P04843
• Protein Accession #: NP_002941
• Nucleotide Accession #: NM_002950.3
• Protein Size (# AA): 607