RPLP0 Protein (OPPA01304)

Catalog No: OPPA01304 (Formerly GWB-82DA5B)

Size:5UG

Product Info

• Predicted Species Reactivity: Human
• Product Format: RPLP0 is supplied at a concentration of 0.9mg/ml in 20mM HEPES buffer pH-7.5, 0.01mM EDTA & 0.02% SDS. Sterile Filtered clear solution.
• Host: Sf9 Insect Cells
• Application: WB
  Immunological Functions: 1. Binds IgG-type human auto-antibodies.2. Standard ELISA test (checker-board analysis of positive/negative sera panels.
  Product Introduction: The ribosomal phosphoproteins, also called P protein antigens, are associated with the large ribosomal subunit and therefore are antigenic targets with a cytoplasmic localization. Three P proteins have been described: P0 with a molecular weight of 35 kDa, P1 (19 kDa) and P2 (17 kDa). RPLP0 is a ribosomal protein that is a component of the 60S subunit. RPLP0 belongs to the L10P family of ribosomal proteins. RPLP0 is a neutral phosphoprotein having a C-terminal end that is nearly identical to the C-terminal ends of the acidic ribosomal phosphoproteins P1 & P2. The P0 protein interacts with P1 and P2 to form a pentameric complex consisting of P1 and P2 dimers, and a P0 monomer. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of RPLP0 scattered throughout the genome.Autoantibodies against ribosomal P proteins are present in 10 % of SLE patients. If anti-ribosomal P antibodies were to occur in the absence of other typical SLE associated autoantibodies, they may account for some patients with so-called ANA-negative lupus. It has been reported that lupus patients positive for anti-ribosomal P autoantibodies have a high frequency of CNS involvement, suggesting a marker use for these antibodies.
  Product Description: Ribosomal Phosphoprotein P0 Human Recombinant produced in SF9 is a glycosylated, polypeptide chain having a molecular mass of 35,096 Dalton. RPLP0 is expressed with a -6xHis tag and purified by proprietary chromatographic techniques.
• Reconstitution and Storage: Store at 4C if entire vial will be used within 2-4 weeks. Store, frozen at -20C for longer periods of time. Avoid multiple freeze-thaw cycles.
• Concentration: Coating concentration: 0.3-0.7 ug/ml (depending on the type of ELISA plate and coating buffer). Suitable for biotinylation and iodination.
• Purity: Greater than 80% as determined by SDS-PAGE.
• Application Info: Western-Blot with monoclonal anti-hexa-His-tag antibody & SLE sera (Systemic Lupus Erythematodes Disease).

Target Info

• Gene Symbol: RPLP0
• Alias Symbols: P0, LP0, L10E, RPP0, PRLP0
• NCBI Gene Id: 6175
• Protein Name: 60S acidic ribosomal protein P0
• Description of Target: Recombinant Human Ribosomal Phosphoprotein P0
• Uniprot ID: P05388
• Protein Accession #: NP_000993.1
• Protein Size (# AA): Recombinant